Identification of mycobacterial species by PCR sequencing of quinolone resistance-determining regions of DNA gyrase genes

被引：42

作者：

Dauendorffer, JN

Guillemin, I

Aubry, A

Truffot-Pernot, C

Sougakoff, W

Jarlier, V

Cambau, E

机构：

[1] Univ Paris 06, Fac Med Pitie Salpetriere, Lab Bacteriol Hyg, F-75634 Paris 13, France

[2] Grp Hosp Pitie Salpetriere, Assistance Publ Hop Paris, Ctr Natl Reference Resistance Mycobacteries Antit, F-75634 Paris, France

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2003年 / 41卷 / 03期

关键词：

D O I：

10.1128/JCM.41.3.1311-1315.2003

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The determination of the amino acid sequence of quinolone resistance-determining regions (QRDRs) in the A and B subunits of DNA gyrase is the molecular test for the detection of fluoroquinolone resistance in mycobacteria. We looked to see if the assignment of mycobacterial species could be obtained simultaneously by analysis of the corresponding nucleotide sequences. PCR sequencing of gyrA and gyrB QRDRs was performed for 133 reference and clinical strains of 21 mycobacterial species commonly isolated in clinical laboratories. Nucleotide sequences of gyrA and gyrB QRDRs were species specific, regardless of fluoroquinolone susceptibility.

引用

页码：1311 / 1315

页数：5

共 41 条

[1] CHARACTERIZATION OF FLUOROQUINOLONE-RESISTANT MUTANT STRAINS OF MYCOBACTERIUM-TUBERCULOSIS SELECTED IN THE LABORATORY AND ISOLATED FROM PATIENTS [J].