Two F420-reducing hydrogenases in Methanosarcina barkeri

被引：23

作者：

Vaupel, M

Thauer, RK ^{[1
]}

机构：

[1] Univ Marburg, Max Planck Inst Terr Mikrobiol, Marburg, Germany

[2] Univ Marburg, Mikrobiol Lab, Fachbereich Biol, Marburg, Germany

来源：

ARCHIVES OF MICROBIOLOGY | 1998年 / 169卷 / 03期

关键词：

methanogenic archaea; Methanosarcina barkeri; hydrogenases; coenzyme F-420; gene expression;

D O I：

10.1007/s002030050561

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

F-420-reducing hydrogenases are nickel iron-sulfur flavoproteins involved in CO2 reduction with H-2 to methane in methanogenic archaea. Evidence is presented that Methanosarcina barkeri contains two isoenzymes for which the encoding genes have been cloned and sequenced. The genes are organized in two operons, frhADGB and freAEGB, each comprising four open reading frames. Transcription analysis revealed that both operons are transcribed during growth of Ms. barkeri on H-2/CO2, on methanol, and on trimethylamine, but not during growth on acetate.

引用

页码：201 / 205

页数：5

共 30 条

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