Sulfation of a squid ink polysaccharide and its inhibitory effect on tumor cell metastasis

被引：129

作者：

Chen, Shiguo ^{[1
]}

Wang, Jingfeng ^{[1
]}

Xue, Changhu ^{[1
]}

Li, Hui ^{[1
]}

Sun, Beibei ^{[1
]}

Xue, Yong ^{[1
]}

Chai, Wengang ^{[2
]}

机构：

[1] Ocean Univ China, Coll Food Sci & Technol, Qingdao 266003, Shandong, Peoples R China

[2] Univ London Imperial Coll Sci Technol & Med, Fac Med, Glycosci Lab, Harrow, Middx, England

来源：

CARBOHYDRATE POLYMERS | 2010年 / 81卷 / 03期

基金：

中国国家自然科学基金;

关键词：

Squid ink polysaccharide; Sulfation; Nuclear magnetic resonance migration; Angiogenesis; MELANIN-FREE INK; STRUCTURAL-CHARACTERIZATION; ANTITUMOR-ACTIVITY; ANTICOAGULANT; PURIFICATION; WATER;

D O I：

10.1016/j.carbpol.2010.03.009

中图分类号：

O69 [应用化学];

学科分类号：

070301 [无机化学];

摘要：

This paper is the first to report the preparation, characterization, and potential biological activities of a chemically sulfated polysaccharide isolated from the ink of the squid, Ommastrephes bartrami. The squid ink polysaccharides (SIPS) were firstly sulfated with the pyridine-sulfur-trioxide complex in dimethyl sulfoxide. Structural characterization of sulfated SIP using nuclear magnetic resonance indicated that sulfation mainly occurred at the 4,6-position of GalNAc. The effects of the sulfated SIP (TBA-1) on tumor cell growth, invasion, and migration were examined in vitro, and its effects on angiogenesis were measured in vivo using the chick embryo chorioallantoic membrane (CAM) assay. TBA-1 did not have any obvious effects on the proliferation of HepG2 tumor cells, but induced the dose-dependent suppression of cell invasion and migration in HepG2. Moreover, TBA-1 obviously inhibited angiogenesis in a CAM model. Thus, our results indicate that TBA-1 is a potential candidate compound for the prevention of tumor metastasis. Crown Copyright (C) 2010 Published by Elsevier Ltd. All rights reserved.

引用

页码：560 / 566

页数：7

共 29 条

[1]

An anticoagulative polysaccharide from an enzymatic hydrolysate of Ecklonia cava [J].