Adventitious root growth and ginsenoside accumulation in Panax ginseng cultures as affected by methyl jasmonate

被引:168
作者
Kim, YS
Hahn, EJ
Murthy, HN
Paek, KY [1 ]
机构
[1] Chungbuk Natl Univ, Res Ctr Dev Adv Hort Technol, Cheongju 361763, South Korea
[2] Chung Ang Univ, Korea Ginseng Inst, Ansung Shi 456756, Kyungii Do, South Korea
[3] Karnatak Univ, Dept Bot, Dharwad 580003, Karnataka, India
关键词
adventitious roots; bioreactor cultures; ginseng; ginsenosides;
D O I
10.1007/s10529-004-3183-2
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 [微生物学]; 0836 [生物工程]; 090102 [作物遗传育种]; 100705 [微生物与生化药学];
摘要
Adventitious roots of ginseng were treated with methyl jasmonate (MJ) up to 150 muM and cultured for 40 days. Up to 100 muM MJ inhibited the root growth but increased ginsenoside accumulation. In a two-stage bioreactor culture, total ginsenosides, after elicitation with 100 muM MJ peaked after 10 days at 48 mg g(-1) dry wt and then dropped sharply. Of the two groups of ginsenosides ( Rb and Rg), higher amounts of Rb accumulated in the adventitious roots.
引用
收藏
页码:1619 / 1622
页数:4
相关论文
共 12 条
[1]
Efficient production of saikosaponins in Bupleurum falcatum root fragments combined with signal transducers [J].
Aoyagi H. ;
Kobayashi Y. ;
Yamada K. ;
Yokoyama M. ;
Kusakari K. ;
Tanaka H. .
Applied Microbiology and Biotechnology, 2001, 57 (4) :482-488
[2]
Hahn Eun-Joo, 2003, Journal of Plant Biotechnology, V5, P1
[3]
Ketchum REB, 1999, BIOTECHNOL BIOENG, V62, P97, DOI 10.1002/(SICI)1097-0290(19990105)62:1&lt
[4]
97::AID-BIT11&gt
[5]
3.0.CO
[6]
2-C
[7]
Elicitation of different Panax ginseng transformed root phenotypes for an improved ginsenoside production [J].
Palazón, J ;
Cusidó, RM ;
Bonfill, M ;
Mallol, A ;
Moyano, E ;
Morales, C ;
Piñol, MT .
PLANT PHYSIOLOGY AND BIOCHEMISTRY, 2003, 41 (11-12) :1019-1025
[8]
SHIN YH, 1997, KORYO INSAM HAKHEOCH, V21, P35
[9]
Production of ginseng and its bioactive components in plant cell culture: Current technological and applied aspects [J].
Wu, JY ;
Zhong, JJ .
JOURNAL OF BIOTECHNOLOGY, 1999, 68 (2-3) :89-99
[10]
Jasmonic acid improves ginsenoside accumulation in adventitious root culture of Panax ginseng CA Meyer [J].
Yu, KW ;
Gao, WY ;
Hahn, EJ ;
Paek, KY .
BIOCHEMICAL ENGINEERING JOURNAL, 2002, 11 (2-3) :211-215