Assembly and overexpression of membrane proteins in Escherichia coli

被引:100
作者
Drew, D [1 ]
Fröderberg, L [1 ]
Baars, L [1 ]
de Gier, JWL [1 ]
机构
[1] Stockholm Univ, Dept Biochem & Biophys, S-10691 Stockholm, Sweden
来源
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES | 2003年 / 1610卷 / 01期
关键词
Escherichia coli; overexpression; membrane protein;
D O I
10.1016/S0005-2736(02)00707-1
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The bacterium Escherichia coli is one of the most popular model systems to study the assembly of membrane proteins of the so-called helix-bundle class. Here, based on this system, we review and discuss what is currently known about the assembly of these membrane proteins. In addition, we will briefly review and discuss how E. coli has been used as a vehicle for the overexpression of membrane proteins. (C) 2003 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:3 / 10
页数:8
相关论文
共 104 条
  • [1] Characterisation of new intracellular membranes in Escherichia coli accompanying large scale over-production of the b subunit of F1Fo ATP synthase
    Arechaga, I
    Miroux, B
    Karrasch, S
    Huijbregts, R
    de Kruijff, B
    Runswick, MJ
    Walker, JE
    [J]. FEBS LETTERS, 2000, 482 (03) : 215 - 219
  • [2] Construction of the 'minimal' SRP that interacts with the translating ribosome but not with specific membrane receptors in Escherichia coli
    Avdeeva, ON
    Myasnikov, AG
    Sergiev, PV
    Bogdanov, AA
    Brimacombe, R
    Dontsova, OA
    [J]. FEBS LETTERS, 2002, 514 (01) : 70 - 73
  • [3] Recombinant protein expression in Escherichia coli
    Baneyx, F
    [J]. CURRENT OPINION IN BIOTECHNOLOGY, 1999, 10 (05) : 411 - 421
  • [4] Discrimination between SRP- and SecA/SecB-dependent substrates involves selective recognition of nascent chains by SRP and trigger factor
    Beck, K
    Wu, LF
    Brunner, J
    Müller, M
    [J]. EMBO JOURNAL, 2000, 19 (01) : 134 - 143
  • [5] YidC, an assembly site for polytopic Escherichia coli membrane proteins located in immediate proximity to the SecYE translocon and lipids
    Beck, K
    Eisner, G
    Trescher, D
    Dalbey, RE
    Brunner, J
    Müller, M
    [J]. EMBO REPORTS, 2001, 2 (08) : 709 - 714
  • [6] Architecture of the protein-conducting channel associated with the translating 80S ribosome
    Beckmann, R
    Spahn, CMT
    Eswar, N
    Helmers, J
    Penczek, PA
    Sali, A
    Frank, J
    Blobel, G
    [J]. CELL, 2001, 107 (03) : 361 - 372
  • [7] The Tat protein export pathway
    Berks, BC
    Sargent, F
    Palmer, T
    [J]. MOLECULAR MICROBIOLOGY, 2000, 35 (02) : 260 - 274
  • [8] Physiological basis for conservation of the signal recognition particle targeting pathway in Escherichia coli
    Bernstein, KD
    Hyndman, JB
    [J]. JOURNAL OF BACTERIOLOGY, 2001, 183 (07) : 2187 - 2197
  • [9] The SecYEG preprotein translocation channel is a conformationally dynamic and dimeric structure
    Bessonneau, P
    Besson, V
    Collinson, I
    Duong, F
    [J]. EMBO JOURNAL, 2002, 21 (05) : 995 - 1003
  • [10] Putative integral membrane SRP receptors
    Bibi, E
    Herskovits, AA
    Bochkareva, ES
    Zelazny, A
    [J]. TRENDS IN BIOCHEMICAL SCIENCES, 2001, 26 (01) : 15 - 16