Affinity capillary electrophoresis

被引：57

作者：

Rippel, G ^{[1
]}

Corstjens, H ^{[1
]}

Billiet, HAH ^{[1
]}

Frank, J ^{[1
]}

机构：

[1] Delft Univ Technol, Dept Biochem Engn, Kluyver Lab Biotechnol, NL-2628 BC Delft, Netherlands

来源：

ELECTROPHORESIS | 1997年 / 18卷 / 12-13期

关键词：

binding constant; affinity capillary electrophoresis; molecular interaction;

D O I：

10.1002/elps.1150181208

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The application of affinity capillary electrophoresis (ACE) to the study of molecular interactions is reviewed. ACE appears to be a sensitive, versatile and convenient tool to obtain reliable data on binding constants and stoichiometries of interacting systems using the Hummel-Dreyer method and variants thereof. A powerful feature is the possibility to analyze simultaneously the affinity of a large number of compounds for the same ligand, making it a promising tool for the screening of large combinatorial libraries.

引用

页码：2175 / 2183

页数：9

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