Retention behavior of large polycyclic aromatic hydrocarbons on cholesteryl 10-undecenoate bonded phase in microcolumn liquid chromatography

The retention behavior of large polycyclic aromatic hydrocarbons (PAH) in reversed-phase microcolumn liquid chromatography has been found to depend primarily on the inherent planarity of the molecules, the degree of orderliness of the bonded phase, and the concentration of solvent. The planarity recognition capability of cholesteryl 10-undecenoate bonded phase for large PAH is comparable with that of monomeric ODS phases. During solvation, however, the phase characteristics seemed to suggest a fairly rigid structure (an ordered phase), i.e. increased selectivity for non-planar molecules which approaches that of polymeric ODS rather than that of other phases with similarly bulky groups, e.g. naphthylethyl and pyrenylethyl bonded phases.