A novel lectin from Sarcophaga -: Its purification, characterization, and cDNA cloning

被引：25

作者：

Fujita, Y ^{[1
]}

Kurata, S ^{[1
]}

Homma, K ^{[1
]}

Natori, S ^{[1
]}

机构：

[1] Univ Tokyo, Fac Pharmaceut Sci, Bunkyo Ku, Tokyo 113, Japan

来源：

JOURNAL OF BIOLOGICAL CHEMISTRY | 1998年 / 273卷 / 16期

关键词：

D O I：

10.1074/jbc.273.16.9667

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A novel C-type lectin that agglutinates rabbit red cells was purified from NIH-Sape-4 cells derived from the flesh fly (Sarcophaga peregrina), and its cDNA was isolated. This lectin, named granulocytin, appeared to be a trimer of a 20-kDa subunit consisting of 151 amino acid residues. The gene for granulocytin was activated in third instar larvae, and its expression was enhanced when the larval body wall was injured. In third instar larvae, granulocytin was found to be synthesized by hemocytes and secreted into the hemolymph. The molecular mass and gene expression patterns of granulocytin were very similar to those of Drosophila lectin that we reported previously (Haq, S., Kubo, T., Kurata, S., Robayashi, A., and Natori, S. (1996) J. Biol. Chem. 271, 20213-20218), However, these two lectins showed amino acid identities of 20% at most, and no significant hapten sugar for granulocytin was identified.

引用

页码：9667 / 9672

页数：6

共 38 条

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