Corneal Epithelial MT1-MMP Inhibits Vascular Endothelial Cell Proliferation and Migration

被引：13

作者：

Azar, Dimitri T. ^{[1
,2
,3
]}

Casanova, Fabio H. ^{[2
,3
]}

Mimura, Tatsuya ^{[1
,2
]}

Jain, Sandeep ^{[1
,3
]}

Zhou, Zhongjun ^{[4
]}

Han, Kyu Yeon ^{[1
]}

Chang, Jin-Hong ^{[1
,2
,3
]}

机构：

[1] Univ Illinois, Dept Ophthalmol & Visual Sci, Chicago, IL 60612 USA

[2] Harvard Univ, Sch Med, Schepens Eye Res Inst, Boston, MA USA

[3] Massachusetts Eye & Ear Infirm, Dept Ophthalmol, Boston, MA 02114 USA

[4] Univ Hong Kong, Dept Biochem, Hong Kong, Hong Kong, Peoples R China

来源：

CORNEA | 2010年 / 29卷 / 03期

基金：

美国国家卫生研究院;

关键词：

cornea; MT1-MMP; TIMP; epithelium; TYPE-1; MATRIX-METALLOPROTEINASE; IN-VITRO; TISSUE INHIBITOR; ANGIOGENESIS; SURFACE; VEGF; NEOVASCULARIZATION; OLIGOMERIZATION; AVASCULARITY; PROTEOLYSIS;

D O I：

10.1097/ICO.0b013e3181b1165d

中图分类号：

R77 [眼科学];

学科分类号：

100212 [眼科学];

摘要：

Purpose: To determine the effects of corneal epithelial membrane-type 1 matrix metalloproteinase (MT1-MMP) on vascular endothelial migration and proliferation. Methods: We generated immortalized wild-type, MT1-MMP knockout and MT1-MMP knock-in corneal epithelial cells. Calf pulmonary arterial endothelial (CPAE) cell proliferation and Boyden chamber migration were assayed. Results: Conditioned media from MT1-MMP epithelial knockout cells significantly increased CPAE proliferation 5-bromo-2'-deoxyuridine (BrdU) incorporation, and CPAE migration as compared with wild-type epithelial cells. Conditioned media from knock-in cells reversed the increase in CPAE proliferation, BrdU incorporation and CPAE migration. Knock-in cells transfected with mutant MT1-MMP (E240A) did not abrogate the reversal effect. Conclusions: Corneal epithelial MT1-MMP is antiangiogenic. This antiangiogenic activity does not require the catalytic domain.

引用

页码：321 / 330

页数：10

共 34 条

[1]

Corneal avascularity is due to soluble VEGF receptor-1 [J].

Ambati, Balamurali K. ;

Nozaki, Miho ;

Singh, Nirbhai ;

Takeda, Atsunobu ;

Jani, Pooja D. ;

Suthar, Tushar ;

Albuquerque, Romulo J. C. ;

Richter, Elizabeth ;

Sakurai, Eiji ;

Newcomb, Michael T. ;

Kleinman, Mark E. ;

Caldwell, Ruth B. ;

Lin, Qing ;

Ogura, Yuichiro ;

Orecchia, Angela ;

Samuelson, Don A. ;

Agnew, Dalen W. ;

St. Leger, Judy ;

Green, W. Richard ;

Mahasreshti, Parameshwar J. ;

Curiel, David T. ;

Kwan, Donna ;

Marsh, Helene ;

Ikeda, Sakae ;

Leiper, Lucy J. ;

Collinson, J. Martin ;

Bogdanovich, Sasha ;

Khurana, Tejvir S. ;

Shibuya, Masabumi ;

Baldwin, Megan E. ;

Ferrara, Napoleone ;

Gerber, Hans-Peter ;

De Falco, Sandro ;

Witta, Jassir ;

Baffi, Judit Z. ;

Raisler, Brian J. ;

Ambati, Jayakrishna .

NATURE, 2006, 443 (7114) :993-997

[2]

Azar Dimitri T, 2006, Trans Am Ophthalmol Soc, V104, P264

[3]

Effect of MT1-MMP Deficiency and Overexpression in Corneal Keratocytes on Vascular Endothelial Cell Migration and Proliferation [J].

Azar, Dimitri T. ;

Casanova, Fabio H. ;

Mimura, Tatsuya ;

Jain, Sandeep ;

Chang, Jin-Hong .

CURRENT EYE RESEARCH, 2008, 33 (11-12) :954-962

[4]

Beaujouin M, 2008, ADV EXP MED BIOL, V617, P453, DOI 10.1007/978-0-387-69080-3_44

[5]

Functional characterization of neostatins, the MMP-derived, enzymatic cleavage products of type XVIII collagen [J].

Chang, JH ;

Javier, JAD ;

Chang, GY ;

Oliveira, HB ;

Azar, DT .

FEBS LETTERS, 2005, 579 (17) :3601-3606

[6]

Genetic and cellular mechanisms of oncogenesis [J].

Coussens, Lisa M. ;

Jacks, Tyler .

CURRENT OPINION IN GENETICS & DEVELOPMENT, 2008, 18 (01) :1-2

[7]

Nonvascular VEGF receptor 3 expression by corneal epithelium maintains avascularity and vision [J].

Cursiefen, Claus ;

Chen, Lu ;

Saint-Geniez, Magali ;

Hamrah, Pedram ;

Jin, Yiping ;

Rashid, Saadia ;

Pytowski, Bronislaw ;

Persaud, Kris ;

Wu, Yan ;

Streilein, J. Wayne ;

Dana, Reza .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2006, 103 (30) :11405-11410

[8]

Vascular endothelial cell growth factor-A - Not just for endothelial cells anymore [J].

D'Amore, Patricia A. .

AMERICAN JOURNAL OF PATHOLOGY, 2007, 171 (01) :14-18

[9]

Anti-angiogenic role of angiostatin during corneal wound healing [J].

Gabison, E ;

Chang, JH ;

Hernández-Quintela, E ;

Javier, J ;

Lu, PCS ;

Ye, HQ ;

Kure, T ;

Kato, T ;

Azar, DT .

EXPERIMENTAL EYE RESEARCH, 2004, 78 (03) :579-589

[10]

Preeclamptic decidual microvascular endothelial cells express lower levels of matrix metalloproteinase-1 than normals [J].

Gallery, EDM ;

Campbell, S ;

Arkell, J ;

Nguyen, M ;

Jackson, CJ .

MICROVASCULAR RESEARCH, 1999, 57 (03) :340-346

← 1 2 3 4 →