A new role for chondrocytes as non-professional phagocytes.: An in vitro study

被引：19

作者：

Cristina, E ^{[1
]}

Castillo, G ^{[1
]}

Kourí, JB ^{[1
]}

机构：

[1] CINVESTAV, IPN, Dept Expt Pathol, Mexico City 14740, DF, Mexico

来源：

MICROSCOPY RESEARCH AND TECHNIQUE | 2004年 / 64卷 / 03期

关键词：

articular cartilage; chondrocytes; apoptosis and phagocytosis;

D O I：

10.1002/jemt.20080

中图分类号：

R602 [外科病理学、解剖学]; R32 [人体形态学];

学科分类号：

100101 ;

摘要：

Chondrocytes are capable of engulfing latex particles, cell detritus, and necrotic and apoptotic remains in vitro. It is conceivable that chondrocytes might be involved in the clearance by phagocytosis of different materials within the cartilage. In fact, so far there is no evidence for the presence of "professional phagocytes" (macrophages and neutrophils) in this tissue. Chondrocyte suspensions obtained from rat knees and hips were cultured to assess phagocytosis of latex particles (1 mum), articular cartilage detritus, and necrotic and apoptotic chondrocyte remains (induced by VP-16 1 mM). We observed that chondrocytes phagocytosed latex particles as evaluated by confocal microscopy and flow cytometry. In addition, we observed that chondrocytes phagocytosed articular cartilage detritus and necrotic and apoptotic VP-16 induced-chondrocytes, as observed by bright field microscopy and transmission electron microscopy. (C) 2004 Wiley-Liss. Inc.

引用

页码：269 / 278

页数：10

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