Standardized molecular typing of Candida albicans strains

被引：8

作者：

Lischewski, A

Harmsen, D

Hacker, J

Morschhauser, J

机构：

[1] Univ Wurzburg, Zentrum Infekt Forsch, D-97070 Wurzburg, Germany

[2] Univ Wurzburg, Inst Hyg & Mikrobiol, D-97070 Wurzburg, Germany

来源：

MYCOSES | 1997年 / 40卷 / 9-10期

关键词：

Candida albicans; molecular typing; DNA fingerprinting; epidemiology;

D O I：

10.1111/j.1439-0507.1997.tb00252.x

中图分类号：

R75 [皮肤病学与性病学];

学科分类号：

100206 ;

摘要：

A method is presented for the standardization of Candida albicans DNA fingerprinting, which is based on Southern hybridization of EcoRI-digested chromosomal DNA with the moderately repetitive DNA element CARE-2 and the subsequent rehybridization of the blots with a molecular size marker also included in each DNA sample. This method resulted in extremely precise alignment of all strain-specific CARE-2 hybridization patterns, even when analysed on different gels, and will enhance the accuracy of genetic relationship determinations in epidemiological studies including large numbers of strains.

引用

收藏

页码：369 / 372

页数：4

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