Immobilized microfluidic enzymatic reactors

被引:200
作者
Krenková, J [1 ]
Foret, F [1 ]
机构
[1] Acad Sci Czech Republ, Inst Analyt Chem, CZ-61142 Brno, Czech Republic
关键词
immobilized enzymes; mass spectrometry; microfluidics; miniaturization; review;
D O I
10.1002/elps.200406096
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
The use of enzymes for cleavage, synthesis or chemical modification represents one of the most common processes used in biochemical and molecular biology laboratories. The continuing progress in medical research, genomics, proteomics, and related emerging biotechnology fields leads to exponential growth of the applications of enzymes and the development of modified or new enzymes with specific activities. Concurrently, new technologies are being developed to improve reaction rates and specificity or perform the reaction in a specific environment. Besides large-scale industrial applications, where typically a large processing capacity is required, there are other, much lower-scale applications, benefiting form the new developments in enzymology. One such technology is microfluidics with the potential to revolutionize analytical instrumentation for the analyses of very small sample amounts, single cells or even subcellular assemblies. This article aims at reviewing the current status of the development of the immobilized microfluidic enzymatic reactors (IMERs) technology.
引用
收藏
页码:3550 / 3563
页数:14
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