Strategies for optimizing heterologous protein expression in Escherichia coli

被引：378

作者：

Hannig, G ^{[1
]}

Makrides, SC ^{[1
]}

机构：

[1] PRAECIS Pharmaceut, Cambridge, MA 02139 USA

来源：

TRENDS IN BIOTECHNOLOGY | 1998年 / 16卷 / 02期

关键词：

D O I：

10.1016/S0167-7799(97)01155-4

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The many advantages of Escherichia coli have ensured that it remains a valuable host for the efficient, cost-effective and high-level production of heterologous proteins. Here, we describe the current status of this prokaryotic expression system and focus on strategies designed to maximize the yields of recombinant proteins. Major challenges facing this expression system are also outlined.

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页码：54 / 60

页数：7

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