In this paper we show for the first time, how junction depths and salicide thicknesses in a 0.25 mu m CMOS process affect the current gain beta of a self-biased lateral NPN transistor, and examine the relationship between beta and the ESD performance. Furthermore, we present a direct method for extracting the self-biased LNPN beta and hence characterize the transistor behavior. Devices with lower beta are found to have lower ESD performance. beta is observed to be strongly influenced by the effective drain/source diffusion depth below the salicide which is determined by the implant energy as well as the amount of active diffusion consumed in silicidation. Substrate resistance as determined by the epitaxial thickness is also shown to be an important parameter defining ESD performance. Possible design trade-offs are discussed.