Infection of human hepatocyte chimeric mouse with genetically engineered hepatitis C virus and its susceptibility to interferon

被引：49

作者：

Hiraga, Nobuhiko

Imamura, Michio

Tsuge, Masataka

Noguchi, Chiemi

Takahashi, Shoichi

Iwao, Eiji

Fujimoto, Yoshifumi

Abe, Hiromi

Maekawa, Toshiro

Ochi, Hidenori

Tateno, Chise

Yoshizato, Katsutoshi

Sakai, Akihito

Sakai, Yoshio

Honda, Masao

Kaneko, Shuichi

Wakita, Takaji

Chayama, Kazuaki

机构：

[1] Hiroshima Univ, Dept Med & Mol Sci, Div Frontier Med Sci, Programs Biomed Res,Grad Sch Biomed Sci,Minami Ku, Hiroshima 7348551, Japan

[2] Hiroshima Univ, Liver Res Project Ctr, Hiroshima 7348551, Japan

[3] Mitsubishi Pharma Corp, Pharmaceut Res Unit, Yokohama, Kanagawa, Japan

[4] RIKEN, Inst Phys & Chem Res, Lab Liver Dis, SNP Res Ctr, Yokohama, Kanagawa, Japan

[5] Prefectural Inst Ind Sci & Technol, Yoshizato Project, CLUSTER, Higashihiroshima, Japan

[6] Hiroshima Univ, Century COE 21, Program Adv Radiat Casualty Med, Dept Biol Sci,Grad Sch Sci, Higashihiroshima 724, Japan

[7] Hiroshima Univ, Dept Biol Lab, Grad Sch Sci, Higashihiroshima 724, Japan

[8] Kanazawa Univ, Dept Gastroenterol, Grad Sch Med, Kanazawa, Ishikawa 920, Japan

来源：

FEBS LETTERS | 2007年 / 581卷 / 10期

关键词：

human hepatocyte chimeric mouse; human serum albumin; HCV RNA; interferon;

D O I：

10.1016/j.febslet.2007.04.021

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We developed a reverse genetics system of hepatitis C virus (HCV) genotypes la and 2a using infectious clones and human hepatocyte chimeric mice. We inoculated cell culture-produced genotype 2a (JFH-1) HCV intravenously. We also injected genotype 1a CV-H77C clone RNA intrahepatically. Mice inoculated with HCV by both procedures developed measurable and transmissible viremia. Interferon (IFN) alpha treatment resulted in greater reduction of genotype 2a HCV levels than genotype la, as seen in clinical practice. Genetically engineered HCV infection system should be useful for analysis of the mechanisms of resistance of HCV to IFN and other drugs. (C) 2007 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：1983 / 1987

页数：5

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