Osmotically relevant membrane signaling complex:: association between HB-EGF, β1-integrin, and CD9 in mTAL

被引:19
作者
Sheikh-Hamad, D
Youker, K
Truong, LD
Nielsen, S
Entman, ML
机构
[1] Baylor Coll Med, Dept Med, Renal Sect, Houston, TX 77030 USA
[2] Baylor Coll Med, Cardiovasc Sci Sect, Houston, TX 77030 USA
[3] Baylor Coll Med, Renal Pathol Lab, Houston, TX 77030 USA
[4] Univ Aarhus, Inst Anat, Dept Cell Biol, DK-8000 Aarhus, Denmark
来源
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY | 2000年 / 279卷 / 01期
关键词
cluster of differentiation-9; heparin-binding epidermal growth factor; beta(1)-integrin; osmotic stress; thick ascending limb; kidney;
D O I
10.1152/ajpcell.2000.279.1.C136
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
The integral membrane proteins cluster of differentiation-9 (CD9), beta(1)-integrin, and heparin-binding epidermal growth factor-like (HB-EGF) exist in association in many cell lines and are linked to intracellular signaling mechanisms. Two of the proteins (CD9 and beta(1)-integrin) are induced by hypertonicity, suggesting that their related signaling processes may be relevant to osmotic stress. The validity of this hypothesis rests upon coexpression and physical association between these molecules in nephron segments that are normally exposed to high and variable ambient osmolality. In this work, we show that CD9 and beta(1)-integrin are induced in rat kidney medulla after dehydration. Immunohistochemistry and immunoprecipitation studies show that CD9, HB-EGF, and beta(1)-integrin are coexpressed and physically associated in medullary thick ascending limbs (mTAL), nephron segments that are normally exposed to high and variable extracellular osmolality. Our findings are consistent with the existence of a cluster of integral membrane proteins in mTAL that may initiate or modulate osmotically relevant signaling pathways.
引用
收藏
页码:C136 / C146
页数:11
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