The Effect of BMP-2 and VEGF Loading of Gelatin-Pectin-BCP Scaffolds to Enhance Osteoblast Proliferation

被引：21

作者：

Amirian, Jhaleh ^{[1
]}

Nguyen Thuy Ba Linh ^{[1
,2
]}

Min, Young Ki ^{[3
]}

Lee, Byong-Taek ^{[1
,2
]}

机构：

[1] Soonchunhyang Univ, Dept Regenerat Med, Coll Med, Cheonan 330090, Chungcheongnam, South Korea

[2] Soonchunhyang Univ, Dept Regenerat Med, Inst Tissue Regenerat, Cheonan 330090, Chungcheongnam, South Korea

[3] Soonchunhyang Univ, Dept Physiol, Coll Med, Cheonan 330090, Chungcheongnam, South Korea

来源：

JOURNAL OF APPLIED POLYMER SCIENCE | 2015年 / 132卷 / 02期

关键词：

biocompatibility; biomaterials; porous materials; TISSUE ENGINEERING APPLICATIONS; DRUG-DELIVERY SYSTEM; BONE REGENERATION; PROTEIN DELIVERY; 3D SCAFFOLD; IN-VITRO; FABRICATION; HYDROGEL; RELEASE; MICROPARTICLES;

D O I：

10.1002/app.41241

中图分类号：

O63 [高分子化学（高聚物）];

学科分类号：

070305 [高分子化学与物理];

摘要：

A composite scaffold of gelatine (Gel)-pectin (Pec)-biphasic calcium phosphate (BCP) was successfully fabricated. Growth factors such as bone morphogenetic protein-2 (BMP-2) and vascular endothelial growth factor (VEGF) were loaded into the Gel-Pec-BCP hydrogel scaffolds by freeze-drying. The surface morphology was investigated by scanning electron microscopy, and BCP dispersion in the hydrogel scaffolds was measured by energy dispersive and X-ray diffraction spectroscopy. The results obtained from Fourier transform infrared spectroscopy and quantitative measurements showed successfully loading of BMP-2 and VEGF into the Gel-Pec-BCP hydrogel scaffolds. In addition MC3T3-E1 preosteoblasts were cultivated on the three types of scaffolds to investigate the effects of BMP-2 and VEGF on cell viability and proliferation. The Gel-Pec-BCP scaffolds loaded with VEGF and BMP-2 demonstrated more cell spreading and proliferation compared to those of the Gel-Pec-BCP scaffolds. (c) 2014 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2015, 132, 41241.

引用

页数：9

共 56 条

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