Expression of the MAL gene in the thyroid:: the MAL proteolipid, a component of glycolipid-enriched membranes, is apically distributed in thyroid follicles

被引:57
作者
Martín-Belmonte, F
Kremer, L
Albar, JP
Marazuela, M
Alonso, MA [1 ]
机构
[1] Univ Autonoma Madrid, Ctr Biol Mol Severo Ochoa, E-28049 Madrid, Spain
[2] CSIC, Ctr Nacl Biotecnol, E-28049 Madrid, Spain
[3] Hosp Princesa, Dept Endocrinol, Madrid 28006, Spain
关键词
D O I
10.1210/en.139.4.2077
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
The MAL proteolipid, an integral membrane protein expressed in T lymphocytes, polarized epithelial MDCK cells, and myelin-forming cells, has been identified as a component of internal glycolipid-enriched membrane (GEM) microdomains. On the basis of its ability to induce vesicle formation by ectopic expression, MAL has been recently proposed as a component of the machinery for GEM vesiculation. Taking into account the proposed role of GEMs in polarized transport, we have investigated the expression of the MAL gene in thyroid cells. Interestingly, MAL messenger RNA species were detected in the human thyroid, whereas they were undetectable in other endocrine glands tested. Moreover, epithelial FRT cells, a polarized rat cell line of thyroid origin, also expressed MAL transcripts. Immunohistochemical analysis of thyroid follicles, with a newly developed anti-MAL monoclonal antibody, indicates that MAL distribution is restricted to the apical zone of thyroid epithelial cells. Biochemical analyses, using FRT cells, indicate exclusive residence of MAL in GEM microdomains, and these analyses allowed the identification of MAL as a major protein component of the GEM fraction in this cell line. Our results are consistent with a role for MAL as a component of GEM microdomains in thyroid epithelial cells.
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页码:2077 / 2084
页数:8
相关论文
共 36 条
[1]   DCNA CLONING AND SEQUENCE OF MAL, A HYDROPHOBIC PROTEIN ASSOCIATED WITH HUMAN T-CELL DIFFERENTIATION [J].
ALONSO, MA ;
WEISSMAN, SM .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (07) :1997-2001
[2]   CULTURE OF HORMONE-DEPENDENT FUNCTIONAL EPITHELIAL-CELLS FROM RAT THYROIDS [J].
AMBESIIMPIOMBATO, FS ;
PARKS, LAM ;
COON, HG .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1980, 77 (06) :3455-3459
[3]  
[Anonymous], 1988, Antibodies: A Laboratory Manual
[4]   SORTING OF GPI-ANCHORED PROTEINS TO GLYCOLIPID-ENRICHED MEMBRANE SUBDOMAINS DURING TRANSPORT TO THE APICAL CELL-SURFACE [J].
BROWN, DA ;
ROSE, JK .
CELL, 1992, 68 (03) :533-544
[5]  
CHOMCZYNSKI P, 1987, ANAL BIOCHEM, V162, P156, DOI 10.1016/0003-2697(87)90021-2
[6]   ISOLATION OF MONOCLONAL-ANTIBODIES SPECIFIC FOR HUMAN C-MYC PROTO-ONCOGENE PRODUCT [J].
EVAN, GI ;
LEWIS, GK ;
RAMSAY, G ;
BISHOP, JM .
MOLECULAR AND CELLULAR BIOLOGY, 1985, 5 (12) :3610-3616
[7]  
GAUSEPOHL H, 1992, PEPTIDE RES, V5, P315
[8]   CLONING AND CHARACTERIZATION OF MVP17 - A DEVELOPMENTALLY-REGULATED MYELIN PROTEIN IN OLIGODENDROCYTES [J].
KIM, T ;
FIEDLER, K ;
MADISON, DL ;
KRUEGER, WH ;
PFEIFFER, SE .
JOURNAL OF NEUROSCIENCE RESEARCH, 1995, 42 (03) :413-422
[9]  
Larsen R.P., 1992, WILLIAMS TXB ENDOCRI, V8, P357
[10]   Baculovirus-based expression of mammalian caveolin in Sf21 insect cells A model system for the biochemical and morphological study of caveolae biogenesis [J].
Li, SW ;
Song, KS ;
Koh, SS ;
Kikuchi, A ;
Lisanti, MP .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1996, 271 (45) :28647-28654