A subset of cytolytic dendritic cells in rat

被引:88
作者
Trinité, B
Voisine, C
Yagita, H
Josien, R
机构
[1] Nantes Univ Hosp, Dept Nephrol & Clin Immunol, F-44093 Nantes 1, France
[2] Juntendo Univ, Sch Med, Dept Immunol, Bunkyo Ku, Tokyo 113, Japan
[3] INSERM Unite 437, Nantes, France
[4] Inst Transplantat & Rech Transplantat, Nantes, France
关键词
D O I
10.4049/jimmunol.165.8.4202
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Dendritic cells (DCs) are a rare population of leukocytes specialized in Ag processing and presentation to T cells, We have previously shown that cultured rat splenic:DCs exhibit a cytotoxic activity against selected target cells. In this study, we analyzed this function in DCs freshly prepared from lymphoid organs using the DC-specific OX62 mAb and magnetic beads, Freshly extracted splenic DCs, but not lymph node and thymic DCs, exhibited a strong and moderate cytotoxic activity against YAC-1 and K562 target cells, respectively, FAGS analyses showed that spleen contained a minor subset (10-15%) of CD4(+) and class IIint DCs that also expressed the OX41 Ag and the lymphoid-related Ags CD5 and CD90 (Thy-1) and a major (80-85%) subset of CD4(-)/OX41(-)/CD5(-) and class IIint DCs, The cytotoxic activity of splenic DCs was strictly restricted to the CD4(-) DCs, a subset poorly represented in LN and thymus, Contrasting with our previous report using cultured splenic DCs, freshly isolated splenic DCs killed YAC-1 cells using a Ca2+-independent mechanism, but this function did not appear mediated by Pas ligand, TNF-related apoptosis-inducing ligand, or TNF-alpha. Therefore, rat DCs contain a subset of naturally cytolytic cells that could play a role in both innate and acquired immune responses. Together with our previous report, these data suggest that rat DCs can use two mechanisms of cytotoxicity depending on their maturation/activation state.
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页码:4202 / 4208
页数:7
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