Celloidin mounting (embedding without infiltration) - a new, simple and reliable method for producing serial sections of high thickness through complete human brains and its application to stereological and immunohistochemical investigations

被引：71

作者：

Heinsen, H

Arzberger, T

Schmitz, C

机构：

[1] Univ Wurzburg, Morphol Brain Res Unit, D-97080 Wurzburg, Germany

[2] Inst Pathol, Dept Neuropathol, D-97080 Wurzburg, Germany

[3] Univ Aachen, RWTH, Dept Anat & Cell Biol, D-52057 Aachen, Germany

来源：

JOURNAL OF CHEMICAL NEUROANATOMY | 2000年 / 20卷 / 01期

关键词：

Alzheimer's disease; human brain; myeloarchitectonics; neuropathology; neuron number; stereology; striatum;

D O I：

10.1016/S0891-0618(00)00067-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Celloidin mounting (embedding without infiltration) of the human central nervous system (CNS) proved to be superior to gelatin embedding for the production of serial sections ranging in thickness from 220 to 500 mum. After gallocyanin-staining, a comprehensive neuroanatomical as well as neuropathological survey of the human brain is possible, including diagnosis of Alzheimer's disease. Details of a fractionator analysis of the total striatal neuron number are described and the possible quantitative analysis of parallel immunohistochemically stained sections is discussed. (C) 2000 Elsevier Science B.V. All rights reserved.

引用

页码：49 / 59

页数：11

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