Sequence analysis and expression of the filamentous phage φLf gene I encoding a 48-kDa protein associated with host cell membrane

One viral strand of phi Lf, a filamentous phage of Xanthomonas campestris pv. campestris, the open reading frame (ORF440) behind gene VI was identified as gene I. This gene codes for pi protein (440 aa, 48 kDa) which was shown to be membrane-bound in the phi Lf-infected host cell by Western blot analysis using the antibody raised against the protein expressed in Escherichia coli. Its predicted amino acid sequence has a nucleotide-binding motif in the N-terminal 97 aa and a membrane-spanning domain (aa 221 to 236). These structural features are characteristic of pIs of several filamentous phages which are transmembrane proteins required for phage assembly. Thus far, nine phi Lf genes have been identified which are organized in the order gII-gX-gV-gVII-gIX-gVIII-gIII-gVI-gI, similar to the genome organization off. coli filamentous phages. (C) 1998 Academic Press.