Inheritance and fine mapping of fertility restoration for cytoplasmic male sterility in Gossypium hirsutum L.

被引：153

作者：

Liu, L ^{[1
]}

Guo, W ^{[1
]}

Zhu, X ^{[1
]}

Zhang, T ^{[1
]}

机构：

[1] Nanjing Agr Univ, Natl Key Lab Crop Genet & Germplasm Enhancement, Cotton Res Inst, Nanjing 210095, Peoples R China

来源：

THEORETICAL AND APPLIED GENETICS | 2003年 / 106卷 / 03期

关键词：

Gossypium hirsutum L; inheritance; molecular mapping; CMS; Rf(1);

D O I：

10.1007/s00122-002-1084-0

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

Genetics of CMS fertility restoration was presented through the analysis of classic genetics and molecular markers. Based on F-2 segregation of the crosses between CMS and the restoring lines, the testcrosses and F-1 x F-1 populations, together with RAPD and SSR mapping, one dominant gene was identified to control the CMS fertility restoration in cotton. The strategy of genotype representation analysis (GRA) was put forward to screen the markers linked with the Rf(1) locus. Using 1,025 random decamer primers and 282 pairs of SSR primers, two RAPD and three SSR markers were identified to be closely linked to the Rf(1) gene. Among the five markers, three were co-dominantly inherited. Additionally, based on the analysis of monosomic and telesomic lines with one SSR maker, the Rf(1) locus could be located on the long arm of chromosome 4. The molecular markers available here are helpful in the development of the elite restoring lines in cotton by marker-assisted selection.

引用

页码：461 / 469

页数：9

共 37 条

[1] Genetics and molecular mapping of a male fertility restoration locus (Rfg1) in rye (Secale cereale L.) [J].