DNA nanoswitch as a biosensor

被引：21

作者：

Buck, Amy H.

Campbell, Colin J. ^{[1
]}

Dickinson, Paul

Mountford, Christopher P.

Stoquert, Helene C.

Terry, Jonathan G.

Evans, Stuart A. G.

Keane, Lorraine M.

Su, Tsueu-Ju

Mount, Andrew R.

Walton, Anthony J.

Beattie, John S.

Crain, Jason

Ghazal, Peter

机构：

[1] Univ Edinburgh, Div Pathway Med, Edinburgh, Midlothian, Scotland

[2] Univ Edinburgh, Sch Phys, Edinburgh, Midlothian, Scotland

[3] Univ Edinburgh, Sch Chem, Edinburgh, Midlothian, Scotland

[4] Univ Edinburgh, Inst Integrated Micro & Nano Syst, Edinburgh, Midlothian, Scotland

来源：

ANALYTICAL CHEMISTRY | 2007年 / 79卷 / 12期

关键词：

D O I：

10.1021/ac070251r

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

We present a new type of DNA switch, based on the Holliday junction, that uses a combination of binding and conformational switching to enable specific label-free detection of DNA and RNA. We show that a single RNA oligonucleotide species can be detected in a complex mixture of extracted cellular RNA and demonstrate that by exploiting different aspects of the switch characteristics we can achieve 30-fold discrimination between single-nucleotide mismatches in a DNA oligonucleotide.

引用

页码：4724 / 4728

页数：5

共 29 条

[1] Spiers Memorial Lecture - Molecular mechanics and molecular electronics [J].