Interaction of thyroxine and estrogen on the expression of estrogen receptor α, cholecystokinin, and preproenkephalin messenger ribonucleic acid in the limbic-hypothalamic circuit

被引:27
作者
Holland, K [1 ]
Norell, A [1 ]
Micevych, P [1 ]
机构
[1] Univ Calif Los Angeles, Sch Med, Dept Neurobiol,Mental Retardat Res Ctr, Neuroendocrinol Lab,Brain Res Inst, Los Angeles, CA 90095 USA
关键词
D O I
10.1210/en.139.3.1221
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
To study thyroid hormone and estrogen interactions in the central nervous system (CNS), the expression of estrogen sensitive genes was examined within the limbic-hypothalamic circuit. Estrogen upregulates the expression of reproductively relevant neuropeptide messenger RNAs (mRNAs) encoding cholecystokinin (CCK) and enkephalin, peptides that stimulate lordosis. Estrogen down-regulates the expression of the estrogen receptor alpha (ER alpha) mRNA in the nuclei of the circuit. We examined the possibility that thyroid hormone treatment would block the estrogen modulation of these messages. Estradiol benzoate (EB), EB + thyroxine (T-4), T-4, or oil were administered to ovariectomized, adult female rats for 10 days. Isotopic situ hybridization histochemistry revealed that within the limbic-hypothalamic nuclei, levels of CCK and preproenkephalin (PPE) mRNA levels were significantly higher in EB and EB + T-4-treated animals compared with T-4 or oil-treated animals. ER alpha mRNA levels were low in EE treated animals, elevated in T-4 or oil-treated animals and further elevated in EB + T-4-treated animals. In summary, T-4 treatment had neither an independent nor an antagonistic effect on estrogen induced expression of CCK or PPE mRNA in the circuit. However, T-4 did prevent the normal estrogenic decrease of ER alpha mRNA levels in the nuclei of the limbic-hypothalamic circuit.
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收藏
页码:1221 / 1228
页数:8
相关论文
共 51 条
[1]   DISTRIBUTION OF PREPROCHOLECYSTOKININ MESSENGER-RNA IN MOTONEURONS OF THE RAT BRAIN-STEM AND SPINAL-CORD [J].
ABELSON, L ;
MICEVYCH, PE .
MOLECULAR BRAIN RESEARCH, 1991, 10 (04) :327-335
[2]   ENDOGENOUS OPIOID-IMMUNOREACTIVE NEURONS OF THE VENTROMEDIAL HYPOTHALAMIC NUCLEUS CONCENTRATE ESTROGEN IN MALE AND FEMALE RATS [J].
AKESSON, TR ;
MICEVYCH, PE .
JOURNAL OF NEUROSCIENCE RESEARCH, 1991, 28 (03) :359-366
[3]   A synthetic oestrogen antagonist, tamoxifen, inhibits oestrogen-induced transcriptional, but not post-transcriptional, regulation of gene expression [J].
Arao, Y ;
Yamamoto, E ;
Miyatake, N ;
Ninomiya, Y ;
Umehara, T ;
Kawashima, H ;
Masushige, S ;
Hasegawa, T ;
Kato, S .
BIOCHEMICAL JOURNAL, 1996, 313 :269-274
[4]   INJECTIONS OF CHOLECYSTOKININ INTO THE VENTROMEDIAL HYPOTHALAMIC NUCLEUS INHIBIT LORDOSIS BEHAVIOR IN THE RAT [J].
BABCOCK, AM ;
BLOCH, GJ ;
MICEVYCH, PE .
PHYSIOLOGY & BEHAVIOR, 1988, 43 (02) :195-199
[5]   ADAPTATION OF MICE TO COLD [J].
BARNETT, SA .
BIOLOGICAL REVIEWS OF THE CAMBRIDGE PHILOSOPHICAL SOCIETY, 1965, 40 (01) :5-&
[6]   INHIBITION OF SEXUAL-BEHAVIOR IN FEMALE RATS BY INTRACEREBRAL INJECTIONS OF MET-ENKEPHALIN IN COMBINATION WITH AN INHIBITOR OF ENKEPHALIN DEGRADING ENZYMES [J].
BEDNAR, I ;
FORSBERG, G ;
SODERSTEN, P .
NEUROSCIENCE LETTERS, 1987, 79 (03) :341-345
[7]   FURTHER EVIDENCE OF NORADRENERGIC REGULATION OF RAT HYPOTHALAMIC ESTROGEN-RECEPTOR CONCENTRATION - POSSIBLE NON-FUNCTIONAL INCREASE AND FUNCTIONAL DECREASE [J].
BLAUSTEIN, JD ;
TURCOTTE, J .
BRAIN RESEARCH, 1987, 436 (02) :253-264
[8]   MAMMALIAN REPRODUCTION - AN ECOLOGICAL PERSPECTIVE [J].
BRONSON, FH .
BIOLOGY OF REPRODUCTION, 1985, 32 (01) :1-26
[9]   EFFECT OF FOOD MANIPULATION ON THE GNRH-LH-ESTRADIOL AXIS OF YOUNG FEMALE RATS [J].
BRONSON, FH .
AMERICAN JOURNAL OF PHYSIOLOGY, 1988, 254 (04) :R616-R621
[10]   DYNAMICS OF INTRACELLULAR ESTROGEN-RECEPTOR REGULATION AS INFLUENCED BY 17BETA-ESTRADIOL [J].
CIDLOWSKI, JA ;
MULDOON, TG .
BIOLOGY OF REPRODUCTION, 1978, 18 (02) :234-246