Transcriptional control of stem cell maintenance in the Drosophila intestine

被引:139
作者
Bardin, Allison J. [1 ]
Perdigoto, Carolina N. [1 ]
Southall, Tony D. [2 ,3 ]
Brand, Andrea H. [2 ,3 ]
Schweisguth, Francois [1 ]
机构
[1] Inst Pasteur, CNRS, URA2578, F-75724 Paris 15, France
[2] Univ Cambridge, Gurdon Inst, Cambridge CB2 1QN, England
[3] Univ Cambridge, Dept Physiol Dev & Neurosci, Cambridge CB2 1QN, England
来源
DEVELOPMENT | 2010年 / 137卷 / 05期
基金
英国惠康基金;
关键词
Drosophila; Notch signaling; Adult stem cells; NOTCH SIGNALING PATHWAY; ORGAN PRECURSOR CELL; SPLIT COMPLEX GENES; MEDIATED REPRESSION; FATE SPECIFICATION; PRONEURAL PROTEINS; DEFAULT REPRESSION; NEURAL DEVELOPMENT; BHLH PROTEINS; WING MARGIN;
D O I
10.1242/dev.039404
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
Adult stem cells maintain tissue homeostasis by controlling the proper balance of stem cell self-renewal and differentiation. The adult midgut of Drosophila contains multipotent intestinal stem cells (ISCs) that self-renew and produce differentiated progeny. Control of ISC identity and maintenance is poorly understood. Here we find that transcriptional repression of Notch target genes by a Hairless-Suppressor of Hairless complex is required for ISC maintenance, and identify genes of the Enhancer of split complex [E(spl)-C] as the major targets of this repression. In addition, we find that the bHLH transcription factor Daughterless is essential to maintain ISC identity and that bHLH binding sites promote ISC-specific enhancer activity. We propose that Daughterless-dependent bHLH activity is important for the ISC fate and that E(spl)-C factors inhibit this activity to promote differentiation.
引用
收藏
页码:705 / 714
页数:10
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