Nonequivalent labeling of the phytyl side chain of chlorophyll a in callus of the hornwort Anthoceros punctatus

被引:16
作者
Itoh, D
Karunagoda, RP
Fushie, T
Katoh, K
Nabeta, K [1 ]
机构
[1] Obihiro Univ Agr & Vet Med, Dept Bioorgan Sci, Obihiro, Hokkaido 0808555, Japan
[2] Shionogi & Co Ltd, Shionogi Res Labs, Aburahi Labs, Shiga 5203423, Japan
来源
JOURNAL OF NATURAL PRODUCTS | 2000年 / 63卷 / 08期
关键词
D O I
10.1021/np000089m
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Callus cultures of the hornwort Anthoceros punctatus were induced from the apical portions of the gametophytes. Calli can accumulate rosmarinic acid, which is suggested as an intermediate for anthocerotonic acid, a rare phenylpropanoid dimer with a cyclobutane ring, indicating that calli possess the ability to produce secondary metabolites found primarily in intact plants. Biosynthesis of chloroplastidic terpenoids of liverworts showed preferential labeling of the farnesyl diphosphate (FPP)-derived portion in the phytyl side chain of chlorophyll a (1) when calli of A. punctatus are incubated with H-2- and C-13-labeled mevalonate. This finding suggests either that cytoplasmic FPP (or isopentenyl diphosphate, IPP) is taken into chloroplasts and condensed with endogenous IPP derived from a nonmevalonate pathway, or that FPP is synthesized within chloroplasts from extraplastidically formed IPP (or mevalonate) and then condensed with endogenous IPP in a different subplastidic fraction.
引用
收藏
页码:1090 / 1093
页数:4
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