MicroRNAs-Proteomic Networks Characterizing Human Medulloblastoma-SLCs

被引：8

作者：

Catanzaro, Giuseppina ^{[1
]}

Besharat, Zein Mersini ^{[2
]}

Garg, Neha ^{[2
]}

Ronci, Maurizio ^{[3
,4
,5
]}

Pieroni, Luisa ^{[3
,6
]}

Miele, Evelina ^{[2
,7
]}

Mastronuzzi, Angela ^{[8
]}

Carai, Andrea ^{[9
]}

Alfano, Vincenzo ^{[2
,7
]}

Po, Agnese ^{[2
]}

Screpanti, Isabella ^{[2
,7
]}

Locatelli, Franco ^{[8
,10
]}

Urbani, Andrea ^{[3
,6
]}

Ferretti, Elisabetta ^{[1
]}

机构：

[1] Univ Roma La Sapienza, Dept Mol Med & Expt Med, I-00161 Rome, Italy

[2] Univ Roma La Sapienza, Dept Mol Med, I-00161 Rome, Italy

[3] Santa Lucia IRCCS Fdn, I-00143 Rome, Italy

[4] Univ G dAnnunzio, Dept Med Oral & Biotechnol Sci, I-66013 Chieti, Italy

[5] Univ S Australia, Mawson Inst, Mawson Lakes, SA 5095, Australia

[6] Univ Roma Tor Vergata, Dept Expt Med & Surg, I-00133 Rome, Italy

[7] Italian Inst Technol, Ctr Life NanoSci Sapienza, Viale Regina Elena 291, I-00161 Rome, Italy

[8] IRCCS, Bambino Gesu Childrens Hosp, Dept Hematol Oncol & Stem Cell Transplantat, I-00165 Rome, Italy

[9] IRCCS, Bambino Gesu Childrens Hosp, Neurosurg Unit, Dept Neurosci & Neurorehabil, I-00165 Rome, Italy

[10] Univ Pavia, Dept Pediat Sci, I-27100 Pavia, Italy

来源：

STEM CELLS INTERNATIONAL | 2016年 / 2016卷

关键词：

CANCER STEM-CELLS; CYCLIN-E; TUMOR; PROGRESSION; SUPPRESSOR; EXPRESSION; MIR-135B; TARGETS;

D O I：

10.1155/2016/2683042

中图分类号：

Q813 [细胞工程];

学科分类号：

100113 [医学细胞生物学];

摘要：

Medulloblastoma (MB) is the most common malignant brain tumor of pediatric age and is characterized by cells expressing stem, astroglial, and neuronal markers. Among them, stem-like cells (hMB-SLCs) represent a fraction of the tumor cell population with the potential of self-renewal and proliferation and have been associated with tumor poor prognosis. In this context, microRNAs have been described as playing a pivotal role in stem cells differentiation. In our paper, we analyze microRNAs profile and genes expression of hMB-SLCs before and after Retinoic Acid- (RA-) induced differentiation. We aimed to identify pivotal players of specific pathways sustaining stemness and/or tumor development and progression and integrate the results of our recent proteomic study. Our results uncovered 22 differentially expressed microRNAs that were used as input together with deregulated genes and proteins in the Genomatix Pathway System (GePS) analysis revealing 3 subnetworks that could be interestingly involved in the maintenance of hMB-SLCs proliferation. Taken together, our findings highlight microRNAs, genes, and proteins that are significantly modulated in hMB-SLCs with respect to their RA-differentiated counterparts and could open new perspectives for prognostic and therapeutic intervention on MB.

引用

页数：10

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