Desiccation and hypertonicity fluid and thermally induced of the airway surface asthma

To determine whether drying and hypertonicity of the airway surface fluid (ASF) are involved in thermally induced asthma, nine subjects performed isocapnic hyperventilation (HV) (minute ventilation 62.2+/-8.3 l/min) of frigid air (-8.9+/-3.3degreesC) while periciliary fluid was collected endoscopically from the trachea. Osmolality was measured by freezing-point depression. The baseline 1-s forced expiratory volume was 73+/-4% of predicted and fell 26.4% 10 min postchallenge (P>0.0001). The volume of ASF collected was 11.0+/-2.2 mul at rest and remained constant during and after HV as the airways narrowed (HV 10.6+/-1.9, recovery 6.5+/-1.7 mul; P=0.18). The osmolality also remained stable throughout (rest 336+/-16, HV 339+/-16, and recovery 352+/-19 mosmol/kgH(2)O, P=0.76). These data demonstrate that airway desiccation and hypertonicity of the ASF do not develop during hyperpnea in asthma; therefore, other mechanisms must cause exercise- and hyperventilation-induced airflow limitation.