Improving DIC microscopy with polarization modulation

被引:11
作者
Holzwarth, G [1 ]
Webb, SC
Kubinski, DJ
Allen, NS
机构
[1] Wake Forest Univ, Dept Phys, Winston Salem, NC 27109 USA
[2] N Carolina State Univ, Dept Bot, Raleigh, NC 27695 USA
来源
JOURNAL OF MICROSCOPY-OXFORD | 1997年 / 188卷
关键词
differential interference contrast microscopy; interference contrast microscopy; polarization modulation; video-enhanced microscopy;
D O I
10.1046/j.1365-2818.1997.2500807.x
中图分类号
TH742 [显微镜];
学科分类号
摘要
It is demonstrated experimentally, as well as analytically, that when the polarization of the light incident upon the first Nomarski-Wollaston prism in a differential interference contrast (DIG) light microscope is switched by 90 degrees, image highlights are changed into shadows and vice versa, Using an inexpensive ferroelectric liquid-crystal modulator, which is easily installed in the microscope, this switching can be done at 30 frames s(-1), synchronized to the camera. Subtraction of alternate digitized frames generates a stream of images in which contrast is doubled, compared with conventional video-enhanced DIG, while image defects and noise tend to cancel. Subtraction of alternate images is carried out efficiently by frame buffer operations and amounts to massively parallel synchronous detection, The new method eliminates the problems inherent in obtaining a separate background image, as required by current video-enhanced DIC practice, without loss of resolution.
引用
收藏
页码:249 / 254
页数:6
相关论文
共 12 条
[1]  
ALIEN RD, 1983, J MICROSC, V129, P3
[2]  
Allen R D, 1969, Z Wiss Mikrosk, V69, P193
[3]   VIDEO-ENHANCED CONTRAST, DIFFERENTIAL INTERFERENCE CONTRAST (AVEC-DIC) MICROSCOPY - A NEW METHOD CAPABLE OF ANALYZING MICROTUBULE-RELATED MOTILITY IN THE RETICULOPODIAL NETWORK OF ALLOGROMIA LATICOLLARIS [J].
ALLEN, RD ;
ALLEN, NS ;
TRAVIS, JL .
CELL MOTILITY AND THE CYTOSKELETON, 1981, 1 (03) :291-302
[4]   LASER SCANNING PHASE MODULATION MICROSCOPE [J].
HANSEN, EW ;
ALLEN, RD ;
STROHBEHN, JW ;
CHAFFEE, MA ;
FARRINGTON, DL ;
MURRAY, WF ;
PILLSBURY, TA ;
RILEY, MF .
JOURNAL OF MICROSCOPY-OXFORD, 1985, 140 :371-381
[5]  
Horowitz P, 1989, ART ELECT
[6]   VIDEO IMAGE-PROCESSING GREATLY ENHANCES CONTRAST, QUALITY, AND SPEED IN POLARIZATION-BASED MICROSCOPY [J].
INOUE, S .
JOURNAL OF CELL BIOLOGY, 1981, 89 (02) :346-356
[7]  
Inoue S., 1986, VIDEO MICROSCOPY
[8]   DESIGN AND APPLICATION OF A COMPUTER-CONTROLLED CONFOCAL SCANNING DIFFERENTIAL POLARIZATION MICROSCOPE [J].
JUANG, CB ;
FINZI, L ;
BUSTAMANTE, CJ .
REVIEW OF SCIENTIFIC INSTRUMENTS, 1988, 59 (11) :2399-2408
[9]  
Lang W., 1968, Nomarski differential interference-contrast microscopy, pp 7082
[10]   SCANNING DIFFERENTIAL POLARIZATION MICROSCOPE - ITS USE TO IMAGE LINEAR AND CIRCULAR DIFFERENTIAL SCATTERING [J].
MICKOLS, W ;
MAESTRE, MF .
REVIEW OF SCIENTIFIC INSTRUMENTS, 1988, 59 (06) :867-872