Lactococcus lactis as a cell factory for high-level diacetyl production

被引：124

作者：

Hugenholtz, J

Kleerebezem, M

Starrenburg, M

Delcour, J

De Vos, W

Hols, P

机构：

[1] NIZO Food Res, Dept Flavour & Nat Ingredients, NL-6710 BA Ede, Netherlands

[2] Wageningen Ctr Food Sci, NL-6710 BA Ede, Netherlands

[3] Univ Catholique Louvain, Mol Genet Lab, B-1348 Louvain, Belgium

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2000年 / 66卷 / 09期

关键词：

D O I：

10.1128/AEM.66.9.4112-4114.2000

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We report the engineering of Lactococcus lactis for the efficient conversion of sugar into diacetyl by combining NADH-oxidase overproduction and alpha-acetolactate decarboxylase inactivation. Eighty percent of the carbon flux was found to be rerouted via alpha-acetolactate to the production of diacetyl by preloading the cells with NADH-oxidase before their use as a cell factory.

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页码：4112 / 4114

页数：3

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