A dual-probe microdialysis study in simultaneously monitoring extracellular pyruvate, lactate, and biogenic amines in gerbil striata during unilateral cerebral ischemia

被引：9

作者：

Cheng, FC

Wang, J

Yang, DY ^{[1
]}

机构：

[1] Taichung Vet Gen Hosp, Dept Emergency, Taichung 407, Taiwan

[2] Chung Shan Med & Dent Coll, Taichung 402, Taiwan

[3] Taichung Vet Gen Hosp, Dept Med Res, Taichung 407, Taiwan

来源：

NEUROCHEMICAL RESEARCH | 2000年 / 25卷 / 08期

关键词：

biogenic amines; cerebral ischemia; dual-probe; gerbil; lactate; microdialysis; pyruvate;

D O I：

10.1023/A:1007618028461

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A dual-probe microdialysis technique coupled with liquid chromatographic assays was developed for the simultaneous monitoring of neurochemicals in gerbil striata during cerebral ischemia. Isocratic separation of lactate and pyruvate was achieved within 5 min whereas the separation of biogenic amines was completed within 30 min. An unilateral ligation was produced by occlusion of the right common carotid artery for 30 mins in anethetized gerbils to perform a typical focal cerebral ischemia. Microdialysis probes were inserted in both sides of the striata to simultaneously monitor biogenic amines, lactate and pyruvate during cerebral ischemia. Dynamic and comparative changes of these analytes in ipsilateral and contralateral sides of the brain can be simultaneously measured by the assay. The present assay can be used as a research tool to explore neurochemical substances and their relationships during cerebral ischemia.

引用

页码：1089 / 1094

页数：6

共 30 条

[1] DIRECT EVIDENCE OF ACUTE, MASSIVE STRIATAL DOPAMINE RELEASE IN GERBILS WITH UNILATERAL STROKES [J].