Practical route to high activity enzyme preparations for synthesis in organic media

被引：78

作者：

Partridge, J ^{[1
]}

Halling, PJ ^{[1
]}

Moore, BD ^{[1
]}

机构：

[1] Univ Strathclyde, Dept Pure & Appl Chem, Glasgow G1 1XL, Lanark, Scotland

来源：

CHEMICAL COMMUNICATIONS | 1998年 / 07期

关键词：

D O I：

10.1039/a800408k

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A single pot method to rapidly prepare immobilised subtilisin Carlsberg and alpha-chymotrypsin gives 1000-fold greater catalytic activities in polar organic solvents than freeze-dried powders.

引用

页码：841 / 842

页数：2

共 24 条

[1] Almarsson O, 1996, BIOTECHNOL BIOENG, V49, P87, DOI 10.1002/(SICI)1097-0290(19960105)49:1<87::AID-BIT11>3.0.CO
[2] 2-8
[3] Biocatalyst behaviour in low-water systems
Bell, G
Halling, PJ
Moore, BD
Partridge, J
Rees, DG
[J]. TRENDS IN BIOTECHNOLOGY, 1995, 13 (11) : 468 - 473
[4] Water activity fails to predict critical hydration level for enzyme activity in polar organic solvents: Interconversion of water concentrations and activities
Bell, G
Janssen, AEM
Halling, PJ
[J]. ENZYME AND MICROBIAL TECHNOLOGY, 1997, 20 (06) : 471 - 477
[5] ACTIVITY AND ENANTIOSELECTIVITY OF SERINE PROTEASES IN TRANSESTERIFICATION REACTIONS IN ORGANIC MEDIA
BROOS, J
ENGBERSEN, JFJ
SAKODINSKAYA, IK
VERBOOM, W
REINHOUDT, DN
[J]. JOURNAL OF THE CHEMICAL SOCIETY-PERKIN TRANSACTIONS 1, 1995, (22): : 2899 - 2905
[6] DRAMATIC ENHANCEMENT OF ENZYMATIC-ACTIVITY IN ORGANIC-SOLVENTS BY LYOPROTECTANTS
DABULIS, K
KLIBANOV, AM
[J]. BIOTECHNOLOGY AND BIOENGINEERING, 1993, 41 (05) : 566 - 571
[7] Enzyme immobilization in MCM-41 molecular sieve
Diaz, JF
Balkus, KJ
[J]. JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC, 1996, 2 (2-3) : 115 - 126
[8] Methanol dramatically enhances serine protease activity under anhydrous conditions
Hutcheon, GA
Parker, MC
James, A
Moore, BD
[J]. CHEMICAL COMMUNICATIONS, 1997, (10) : 931 - 932
[9] Hutcheon GA, 1997, METHOD ENZYMOL, V286, P465
[10] AQUEOUS-LIKE ACTIVITY OF ALPHA-CHYMOTRYPSIN DISSOLVED IN NEARLY ANHYDROUS ORGANIC-SOLVENTS
PARADKAR, VM
DORDICK, JS
[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1994, 116 (11) : 5009 - 5010

← 1 2 3 →