Conditions for open-air outdoor culture of Dunaliella salina in southern Spain

被引:67
作者
García-González, M
Moreno, J
Cañavate, JP
Anguis, V
Prieto, A
Manzano, C
Florencio, FJ
Guerrero, MG
机构
[1] Univ Seville, CSIC, Inst Bioquim Vegetal & Fotosintesis, Seville 41092, Spain
[2] Ctr Invest & Cultivo Especies Marinas El Toruno, Cadiz 11500, Spain
[3] Consejeria Agr & Pesca, Seville 41013, Spain
关键词
beta-carotene; andalusian coast; biotechnology; Dunaliella salina; microalga; outdoor culture;
D O I
10.1023/A:1023892520443
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The optimization of the operation, under the climatic conditions of southern Spain, of an experimental plant for beta-carotene production by Dunaliella has been pursued. The effects of mixing, culture depth, cell density and dilution cycles on beta-carotene and biomass productivity were studied under a semicontinuous culture regime in open tanks outdoors. Using 3 m(2)-surface containers, the highest productivity values, for both beta-carotene and biomass, were recorded with a flow rate of 0.55 m s(-1); 10 cm depth; 0.7-0.9x10(6) cell ml(-1), population density; and dilution cycles of two days. An average annual productivity of 1.65 g (dry wt) m(-2) d(-1) was estimated for Dunaliella biomass, being that for beta-carotene of about 0.1 g m(-2) d(-1). Under these optimized conditions, experiments have been carried out at the Cadiz Bay with 20 m(2)-surface tanks during a whole-year cycle. The results obtained have validated this location and the operating conditions established as being most appropriate for efficient mass production of beta-carotene rich D. salina.
引用
收藏
页码:177 / 184
页数:8
相关论文
共 20 条
[1]  
Abalde, 1992, PROFILES BIOTECHNOLO, P301
[2]  
Ben-Amatoz A, 1989, ALGAL CYANOBACTERIAL, P90
[3]  
Ben-Amotz Ami, 1999, P401
[4]   NEW MODE OF DUNALIELLA BIOTECHNOLOGY - 2-PHASE GROWTH FOR BETA-CAROTENE PRODUCTION [J].
BENAMOTZ, A .
JOURNAL OF APPLIED PHYCOLOGY, 1995, 7 (01) :65-68
[5]  
Borowitzka L. J., 1992, PROFILES BIOTECHNOLO, P233
[6]  
BOROWITZKA M A, 1990, Journal of Applied Phycology, V2, P111, DOI 10.1007/BF00023372
[7]   Commercial production of microalgae: ponds, tanks, tubes and fermenters [J].
Borowitzka, MA .
JOURNAL OF BIOTECHNOLOGY, 1999, 70 (1-3) :313-321
[8]   GROWTH AND CAROTENOGENESIS IN 8 STRAINS OF DUNALIELLA-SALINA TEODORESCO FROM CHILE [J].
CIFUENTES, AS ;
GONZALEZ, M ;
CONEJEROS, M ;
DELLAROSSA, V ;
PARRA, O .
JOURNAL OF APPLIED PHYCOLOGY, 1992, 4 (02) :111-118
[9]   The carotenoids as anti-oxidants - a review [J].
Edge, R ;
McGarvey, DJ ;
Truscott, TG .
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY, 1997, 41 (03) :189-200
[10]   STUDIES OF THE COMPARATIVE PHYSIOLOGY OF THE GENUS DUNALIELLA (CHLOROPHYTA, VOLVOCALES) .1. RESPONSE OF GROWTH TO NACL CONCENTRATION [J].
GINZBURG, BZ ;
GINZBURG, M .
BRITISH PHYCOLOGICAL JOURNAL, 1985, 20 (03) :277-283