Detection of low-copy-number genomic DNA sequences in individual bacterial cells by using peptide nucleic acid-assisted rolling-circle amplification and fluorescence in situ hybridization

被引：36

作者：

Smolina, Irina

Lee, Charles

Frank-Kamenetskii, Maxim

机构：

[1] Boston Univ, Ctr Adv Biotechnol, Boston, MA 02215 USA

[2] Boston Univ, Dept Biomed Engn, Boston, MA 02215 USA

[3] Harvard Univ, Brigham & Womens Hosp, Sch Med, Dept Pathol, Boston, MA 02115 USA

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2007年 / 73卷 / 07期

关键词：

D O I：

10.1128/AEM.02038-06

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

An approach is proposed for in situ detection of short signature DNA sequences present in single copies per bacterial genome. The site is locally opened by peptide nucleic acids, and a circular oligonucleotide is assembled. The amplicon generated by rolling circle amplification is detected by hybridization with fluorescently labeled decorator probes.

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页码：2324 / 2328

页数：5

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