Phosphorus J coupling constants of ATP in human myocardium and calf muscle

被引：13

作者：

Jung, WI ^{[1
]}

Widmaier, S ^{[1
]}

Seeger, U ^{[1
]}

Bunse, M ^{[1
]}

Staubert, A ^{[1
]}

Sieverding, L ^{[1
]}

Straubinger, K ^{[1
]}

vanErckelens, F ^{[1
]}

Schick, F ^{[1
]}

Dietze, G ^{[1
]}

Lutz, O ^{[1
]}

机构：

[1] UNIV TUBINGEN, INST PHYS, D-72076 TUBINGEN, GERMANY

来源：

JOURNAL OF MAGNETIC RESONANCE SERIES B | 1996年 / 110卷 / 01期

关键词：

D O I：

10.1006/jmrb.1996.0005

中图分类号：

O64 [物理化学（理论化学）、化学物理学]; O56 [分子物理学、原子物理学];

学科分类号：

070203 ; 070304 ; 081704 ; 1406 ;

摘要：

Proton-decoupled P-31 NMR spectroscopy of the heart and calf muscle of healthy volunteers was performed with a 1.5 T whole-body imager. By use of two-dimensional chemical-shift imaging in combination with slice-selective excitation, well-resolved localized spectra (elements of 38 mi) were obtained within 20 to 35 min from which the homonuclear J coupling constants of ATP could be determined. In myocardium, J(gamma beta) = 16.03 +/- 0.17 Hz and J(alpha beta) = 15.82 +/- 0.23 Hz were obtained, while the values in calf muscle were J(gamma beta) = 17.16 +/- 0.12 Hz and J(alpha beta) = 16.04 +/- 0.09 Hz, The difference in J(gamma beta) was significant. According to the literature, a possible reason for greater ATP J coupling constants is a smaller fraction of ATP complexed to magnesium. However, the chemical-shift difference between alpha- and beta-ATP, which is also a measure for the fraction of ATP complexed to magnesium, showed only a small difference in ATP complexation: 88% in myocardium and 90% in calf muscle, This small difference cannot account for the observed difference in J gamma beta. (C) 1996 Academic Press, Inc.

引用

页码：39 / 46

页数：8

共 38 条

[1] PROBLEMS IN THE ASSESSMENT OF MAGNESIUM DEPLETION IN THE RAT BY INVIVO P-31 NMR [J].