Bloom of filamentous bacteria in a mesotrophic lake:: Identity and potential controlling mechanism

被引:74
作者
Pernthaler, J
Zöllner, E
Warnecke, F
Jürgens, K
机构
[1] Max Planck Inst Marine Mikrobiol, D-28359 Bremen, Germany
[2] Max Planck Inst Limnol, D-2320 Plon, Germany
[3] Baltic Sea Res Inst, Warnemunde, Germany
关键词
D O I
10.1128/AEM.70.10.6272-6281.2004
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Ephemeral blooms of filamentous bacteria are a common phenomenon in the water column of oligo- to mesotrophic lakes. It is assumed that the appearance of such morphotypes is favored by selective predation of bacterivorous protists and that filter-feeding zooplankton plays a major role in suppressing these bacteria. The pbylogenetic affiliation of the important bloom-forming filamentous bacteria in freshwaters is presently unknown. Here we report the identification of dominant members of a filamentous bacterial assemblage during a bloom of such morphotypes in a mesotrophic lake. By molecular cloning and fluorescence in situ hybridization with specific oligonucleotide probes, up to 98% of filamentous cells in lake water could be assigned to a clade of almost identical (99% similarity) 16S rRNA gene sequence types, the cosmopolitan freshwater LD2 cluster. For a period of less than 1 week, members of the LD2 clade constituted >40% of the total bacterial biomass, potentially favored by high grazing of planktivorous protists. This is probably the most pronounced case of dominance by a single bacterioplankton species ever observed in natural freshwaters. In enclosures artificially stocked with the metazoan filter feeder Daphnia, bacteria related to the LD2 clade formed a significantly larger fraction of filaments than in enclosures where Daphnia had been removed. However, in the presence of higher numbers of Daphnia individuals, the LD2 bacteria, like other filaments, were eventually eliminated both in enclosures and in the lake. This points at the potential importance of filter-feeding zooplankton in controlling the occurrence and species composition of filamentous bacterial morphotypes in freshwater plankton.
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页码:6272 / 6281
页数:10
相关论文
共 55 条
[1]   PHYLOGENETIC IDENTIFICATION AND IN-SITU DETECTION OF INDIVIDUAL MICROBIAL-CELLS WITHOUT CULTIVATION [J].
AMANN, RI ;
LUDWIG, W ;
SCHLEIFER, KH .
MICROBIOLOGICAL REVIEWS, 1995, 59 (01) :143-169
[2]  
Bell R. T., 1993, HDB METHODS AQUATIC, P495
[4]   FILTER MESH SIZE OF CLADOCERANS PREDICTS RETENTION EFFICIENCY FOR BACTERIA [J].
BRENDELBERGER, H .
LIMNOLOGY AND OCEANOGRAPHY, 1991, 36 (05) :884-894
[5]   The domain-specific probe EUB338 is insufficient for the detection of all Bacteria:: Development and evaluation of a more comprehensive probe set [J].
Daims, H ;
Brühl, A ;
Amann, R ;
Schleifer, KH ;
Wagner, M .
SYSTEMATIC AND APPLIED MICROBIOLOGY, 1999, 22 (03) :434-444
[6]   Rapid Daphnia-mediated changes in microbial community structure:: an experimental study [J].
Degans, H ;
Zöllner, E ;
Van der Gucht, K ;
De Meester, L ;
Jürgens, K .
FEMS MICROBIOLOGY ECOLOGY, 2002, 42 (01) :137-149
[7]   Flow cytometric determination of bacterial abundance in lake plankton with the green nucleic acid stain SYTO 13 [J].
delGiorgio, P ;
Bird, DF ;
Prairie, YT ;
Planas, D .
LIMNOLOGY AND OCEANOGRAPHY, 1996, 41 (04) :783-789
[8]   HOW CLOSE IS CLOSE - 16S RIBOSOMAL-RNA SEQUENCE IDENTITY MAY NOT BE SUFFICIENT TO GUARANTEE SPECIES IDENTITY [J].
FOX, GE ;
WISOTZKEY, JD ;
JURTSHUK, P .
INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY, 1992, 42 (01) :166-170
[9]  
Fuchs BM, 1998, APPL ENVIRON MICROB, V64, P4973
[10]  
Gasol JM, 1999, APPL ENVIRON MICROB, V65, P4475