Using poly(lactide-co-glycolide) electrospun scaffolds to deliver cultured epithelial cells to the cornea

被引：49

作者：

Deshpande, Pallavi ^{[1
]}

McKean, Rob ^{[2
]}

Blackwood, Keith A. ^{[1
]}

Senior, Richard A. ^{[1
]}

Ogunbanjo, Adekemi ^{[1
]}

Ryan, Anthony J. ^{[2
]}

MacNeil, Sheila ^{[1
]}

机构：

[1] Univ Sheffield, Dept Mat Engn, Kroto Res Inst, Sheffield S3 7HQ, S Yorkshire, England

[2] Univ Sheffield, Dept Chem, Sheffield S3 7HF, S Yorkshire, England

来源：

REGENERATIVE MEDICINE | 2010年 / 5卷 / 03期

关键词：

cell carrier; cornea electrospun; limbal; poly(lactide-co-glycolide); LIMBAL STEM-CELLS; AMNIOTIC MEMBRANE TRANSPLANTATION; SURFACE RECONSTRUCTION; IMMUNOGENICITY; DEGRADATION; CULTIVATION; PROTEINS; FIBRIN;

D O I：

10.2217/RME.10.16

中图分类号：

Q813 [细胞工程];

学科分类号：

100113 [医学细胞生物学];

摘要：

Aims: To assess the potential of electrospun poly(lactide-co-glycolide) membranes to provide a biodegradable cell carrier system for limbal epithelial cells. Material & methods: 50:50 poly(lactide-co-glycolide) scaffolds were spun, sterilized and seeded with primary rabbit limbal epithelial cells. Cells were cultured on the scaffolds for 2 weeks and then examined by confocal microscopy, cryosectioning and scanning-electron microscopy. The tensile strength of scaffolds before and after annealing and sterilization was also studied. Results: The limbal cells had formed a continuous multilayer of cells on either side of the scaffold. Scaffolds with cells showed signs of the onset of degradation within 2 weeks in culture media at 37 degrees C. Scaffolds that were annealed resulted in a more brittle and stiff mat. Conclusions: We suggest this carrier membrane could be used as a replacement for the human amniotic membrane in the treatment of limbal stem cell deficiency, lowering the risk of disease transmission to the patient.

引用

页码：395 / 401

页数：7

共 37 条

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