Development of a PCR test based on a gene region associated with the pathogenicity of Pasteurella multocida serotype B:2, the causal agent of haemorrhagic septicaemia in Asia

被引:19
作者
Brickell, SK [1 ]
Thomas, LM [1 ]
Long, KA [1 ]
Panaccio, M [1 ]
Widders, PR [1 ]
机构
[1] Victorian Inst Anim Sci, Attwood, Vic 3049, Australia
关键词
haemorrhagic septicaemia; Pasteurella multocida; PCR; diagnosis bacteria;
D O I
10.1016/S0378-1135(97)00199-5
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
We have developed a PCR assay to detect Pasteurella multocida serotype B:2, the causal agent of Haemorrhagic Septicaemia (HS) in Asia. Nucleotide sequence determination of a 16S rRNA-23S rRNA PCR product unique to B:2 strains was shown to share amino acid sequence homology with a bacteriophage Mu protein. Primers designed from this sequence when tested against a panel of isolates recovered from a wide geographical area and representing a large range of bacterial genera and species, were found to specifically amplify DNA from P. multocida, serotype B:2. Southern hybridisation confirmed the presence of this sequence in only the B:2 serotype of P. multocida, suggesting an association between bacterial virulence and the presence of bacteriophage genes in the bacterial genome. The results of this study demonstrate the potential application of PCR to the diagnosis of HS in cattle and buffalo in Asia. Application of PCR to support diagnosis of HS will greatly improve accuracy, laboratory response time, and will facilitate rational deployment of resources for controlling this disease. (C) 1998 Elsevier Science B.V.
引用
收藏
页码:295 / 307
页数:13
相关论文
共 12 条
[1]  
[Anonymous], 1989, Pasteurella and pasteurellosis
[2]  
Ausubel FM, 1995, CURRENT PROTOCOLS MO
[3]  
Baxa C A, 1992, DNA Seq, V2, P329, DOI 10.3109/10425179209030967
[4]   A ROLE FOR BACTERIOPHAGES IN THE EVOLUTION AND TRANSFER OF BACTERIAL VIRULENCE DETERMINANTS [J].
CHEETHAM, BF ;
KATZ, ME .
MOLECULAR MICROBIOLOGY, 1995, 18 (02) :201-208
[5]   RAPID IDENTIFICATION OF PASTEURELLA-MULTOCIDA ORGANISMS RESPONSIBLE FOR HEMORRHAGIC SEPTICEMIA USING AN ENZYME-LINKED-IMMUNOSORBENT-ASSAY [J].
DAWKINS, HJS ;
JOHNSON, RB ;
SPENCER, TL ;
PATTEN, BE .
RESEARCH IN VETERINARY SCIENCE, 1990, 49 (03) :261-267
[6]  
DEALWIS MCL, 1992, BRIT VET J, V148, P99, DOI 10.1016/0007-1935(92)90101-6
[7]   WHOLE-GENOME RANDOM SEQUENCING AND ASSEMBLY OF HAEMOPHILUS-INFLUENZAE RD [J].
FLEISCHMANN, RD ;
ADAMS, MD ;
WHITE, O ;
CLAYTON, RA ;
KIRKNESS, EF ;
KERLAVAGE, AR ;
BULT, CJ ;
TOMB, JF ;
DOUGHERTY, BA ;
MERRICK, JM ;
MCKENNEY, K ;
SUTTON, G ;
FITZHUGH, W ;
FIELDS, C ;
GOCAYNE, JD ;
SCOTT, J ;
SHIRLEY, R ;
LIU, LI ;
GLODEK, A ;
KELLEY, JM ;
WEIDMAN, JF ;
PHILLIPS, CA ;
SPRIGGS, T ;
HEDBLOM, E ;
COTTON, MD ;
UTTERBACK, TR ;
HANNA, MC ;
NGUYEN, DT ;
SAUDEK, DM ;
BRANDON, RC ;
FINE, LD ;
FRITCHMAN, JL ;
FUHRMANN, JL ;
GEOGHAGEN, NSM ;
GNEHM, CL ;
MCDONALD, LA ;
SMALL, KV ;
FRASER, CM ;
SMITH, HO ;
VENTER, JC .
SCIENCE, 1995, 269 (5223) :496-512
[8]   A UNIVERSAL APPROACH TO BACTERIAL MOLECULAR EPIDEMIOLOGY BY POLYMERASE CHAIN-REACTION RIBOTYPING [J].
KOSTMAN, JR ;
ALDEN, MB ;
MAIR, M ;
EDLIND, TD ;
LIPUMA, JJ ;
STULL, TL .
JOURNAL OF INFECTIOUS DISEASES, 1995, 171 (01) :204-208
[9]   MOLECULAR EPIDEMIOLOGY OF PSEUDOMONAS-CEPACIA DETERMINED BY POLYMERASE CHAIN-REACTION RIBOTYPING [J].
KOSTMAN, JR ;
EDLIND, TD ;
LIPUMA, JJ ;
STULL, TL .
JOURNAL OF CLINICAL MICROBIOLOGY, 1992, 30 (08) :2084-2087
[10]  
Maniatis T., 1982, MOL CLONING A LAB MA