Aldehyde dehydrogenase 6, a cytosolic retinaldehyde dehydrogenase prominently expressed in sensory neuroepithelia during development

被引:109
作者
Grün, F
Hirose, Y
Kawauchi, S
Ogura, T
Umesono, K
机构
[1] Kyoto Univ, Inst Virus Res, Kyoto 6068507, Japan
[2] Univ Tsukuba, Inst Basic Med Sci, Tsukuba, Ibaraki 3058575, Japan
[3] Nara Inst Sci & Technol, Ikoma 6300101, Japan
关键词
D O I
10.1074/jbc.M007376200
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We have isolated the chick and mouse homologs of human aldehyde dehydrogenase 6 (ALDH6) that encode a third cytosolic retinaldehyde-specific aldehyde dehydrogenase, In both chick and mouse embryos, strong expression is observed in the sensory neuroepithelia of the head. In situ hybridization analysis in chick, shows compartmentalized expression primarily in the ventral retina, olfactory epithelium, and otic vesicle; additional sites of expression include the isthmus, Rathke's pouch, posterior spinal cord interneurons, and developing limbs. Recombinant chick ALDH6 has a K-0.5 = 0.26 muM, V-max = 48.4 nmol/min/mg and exhibits strong positive cooperativity (H = 1.9) toward all-trans-retinaldehyde; mouse ALDH6 has similar kinetic parameters. Expression constructs can confer 1000-fold increased sensitivity to retinoic acid receptor-dependent signaling from retinol in transient transfections experiments. The localization of ALDH6 to the developing sensory neuroepithelia of the eye, nose, and ear and discreet sites within the CNS suggests a role for RA signaling during primary neurogenesis at these sites.
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收藏
页码:41210 / 41218
页数:9
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