Auxin influx activity is associated with Frankia infection during actinorhizal nodule formation in Casuarina glauca

被引:58
作者
Peret, Benjamin
Swarup, Ranjan
Jansen, Leen
Devos, Gaelle
Auguy, Florence
Collin, Myriam
Santi, Carole
Hocher, Valerie
Franche, Claudine
Bogusz, Didier
Bennett, Malcolm
Laplaze, Laurent [1 ]
机构
[1] Univ Montpellier 2, Inst Rech Pour Dev, Inst Natl Rech Agronom, Unite Mixte Rech Diversite& Adapt Plantes Cultive, Montpellier 5, France
[2] Univ Nottingham, Sch Biol Sci, Div Plant Sci, Nottingham NG7 2RD, England
基金
英国生物技术与生命科学研究理事会;
关键词
D O I
10.1104/pp.107.101337
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Plants from the Casuarinaceae family enter symbiosis with the actinomycete Frankia leading to the formation of nitrogen-fixing root nodules. We observed that application of the auxin influx inhibitor 1-naphtoxyacetic acid perturbs actinorhizal nodule formation. This suggests a potential role for auxin influx carriers in the infection process. We therefore isolated and characterized homologs of the auxin influx carrier (AUX1-LAX) genes in Casuarina glauca. Two members of this family were found to share high levels of deduced protein sequence identity with Arabidopsis (Arabidopsis thaliana) AUX-LAX proteins. Complementation of the Arabidopsis aux1 mutant revealed that one of them is functionally equivalent to AUX1 and was named CgAUX1. The spatial and temporal expression pattern of CgAUX1 promoter:beta-glucuronidase reporter was analyzed in Casuarinaceae. We observed that CgAUX1 was expressed in plant cells infected by Frankia throughout the course of actinorhizal nodule formation. Our data suggest that auxin plays an important role during plant cell infection in actinorhizal symbioses.
引用
收藏
页码:1852 / 1862
页数:11
相关论文
共 50 条
[1]   PHYTOHORMONES, RHIZOBIUM MUTANTS, AND NODULATION IN LEGUMES .3. AUXIN METABOLISM IN EFFECTIVE AND INEFFECTIVE PEA ROOT-NODULES [J].
BADENOCHJONES, J ;
ROLFE, BG ;
LETHAM, DS .
PLANT PHYSIOLOGY, 1983, 73 (02) :347-352
[2]   Arabidopsis AUX1 gene: A permease-like regulator of root gravitropism [J].
Bennett, MJ ;
Marchant, A ;
Green, HG ;
May, ST ;
Ward, SP ;
Millner, PA ;
Walker, AR ;
Schulz, B ;
Feldmann, KA .
SCIENCE, 1996, 273 (5277) :948-950
[3]   BIOLOGY OF FRANKIA STRAINS, ACTINOMYCETE SYMBIONTS OF ACTINORHIZAL PLANTS [J].
BENSON, DR ;
SILVESTER, WB .
MICROBIOLOGICAL REVIEWS, 1993, 57 (02) :293-319
[4]  
Berg RH, 1999, CAN J BOT, V77, P1351, DOI 10.1139/cjb-77-9-1351
[5]  
Berg RH, 1999, CAN J BOT, V77, P1327, DOI 10.1139/cjb-77-9-1327
[6]   Flavonoids act as negative regulators of auxin transport in vivo in Arabidopsis [J].
Brown, DE ;
Rashotte, AM ;
Murphy, AS ;
Normanly, J ;
Tague, BW ;
Peer, WA ;
Taiz, L ;
Muday, GK .
PLANT PHYSIOLOGY, 2001, 126 (02) :524-535
[7]   Dissecting Arabidopsis lateral root development [J].
Casimiro, I ;
Beeckman, T ;
Graham, N ;
Bhalerao, R ;
Zhang, HM ;
Casero, P ;
Sandberg, G ;
Bennett, MJ .
TRENDS IN PLANT SCIENCE, 2003, 8 (04) :165-171
[8]  
Cérémonie H, 1999, CAN J BOT, V77, P1293, DOI 10.1139/cjb-77-9-1293
[9]   ISOLATION OF BIOLOGICALLY-ACTIVE RIBONUCLEIC-ACID FROM SOURCES ENRICHED IN RIBONUCLEASE [J].
CHIRGWIN, JM ;
PRZYBYLA, AE ;
MACDONALD, RJ ;
RUTTER, WJ .
BIOCHEMISTRY, 1979, 18 (24) :5294-5299
[10]   Floral dip:: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana [J].
Clough, SJ ;
Bent, AF .
PLANT JOURNAL, 1998, 16 (06) :735-743