OsWRKY13 mediates rice disease resistance by regulating defense-related genes in salicylate- and jasmonate-dependent signaling

被引：392

作者：

Qiu, Deyun ^{[1
]}

Xiao, Jun ^{[1
]}

Ding, Xinhua ^{[1
]}

Xiong, Min ^{[1
]}

Cai, Meng ^{[1
]}

Cao, Yinglong ^{[1
]}

Li, Xianghua ^{[1
]}

Xu, Caiguo ^{[1
]}

Wang, Shiping ^{[1
]}

机构：

[1] Huazhong Agr Univ, Natl Key Lab Crop Genet Improvement, Natl Ctr Plant Gene Res, Wuhan 430070, Peoples R China

来源：

MOLECULAR PLANT-MICROBE INTERACTIONS | 2007年 / 20卷 / 05期

关键词：

Oryza sativa;

D O I：

10.1094/MPMI-20-5-0492

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Although 109 WRKY genes have been identified in the rice genome, the functions of most are unknown. Here, we show that OsWRKY13 plays a pivotal role in rice disease resistance. Overexpression of OsWRKY13 can enhance rice resistance to bacterial blight and fungal blast, two of the most devastating diseases of rice worldwide, at both the seedling and adult stages, and shows no influence on the fertility. This overexpression was accompanied by the activation of salicylic acid (SA) synthesis-related genes and SA-responsive genes and the suppression of jasmonic acid (JA) synthesis-related genes and JA-responsive genes. OsWRKY13 bound to the promoters of its own and at least three other genes in SA- and JA-dependent signaling pathways. Its DNA-binding activity was influenced by pathogen infection. These results suggest that OsWRKY13, as an activator of the SA-dependent pathway and a suppressor of JA-dependent pathways, mediates rice resistance by directly or indirectly regulating the expression of a subset of genes acting both upstream and downstream of SA and JA. Furthermore, OsWRKY13 will provide a transgenic tool for engineering wider-spectrum and whole-growth-stage resistance rice in breeding programs.

引用

页码：492 / 499

页数：8

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[1] A novel rice (Oryza sativa L.) acidic PR1 gene highly responsive to cut, phytohormones, and protein phosphatase inhibitors [J].