A novel mutation in the α-helix 1 of the C subunit of the F1/F0 ATPase responsible for optochin resistance of a Streptococcus pneumoniae clinical isolate

Previously reported mutations involved in optochin resistance of Streptococcus pneumoniae clinical isolates changed residues 48, 49 or 50, in the transmembrane alpha-helix 2 of the F-1/F-0 ATPase subunit. We report here an unusual mutation which changes the sequence of the transmembrane alpha-helix 1 of the AtpC subunit. This mutation involves a Gly to Ser substitution resulting from a G to A transition at codon 14 of the atpC gene. (C) 2000 Elsevier Science Inc. All rights reserved.