Cu, Zn-superoxide dismutase of Saccharomyces cerevisiae is required for resistance to hyperosmosis

被引：22

作者：

Garay-Arroyo, A

Lledías, F

Hansberg, W

Covarrubias, AA

机构：

[1] Inst Biotechnol, Dept Biol Mol Plantas, Cuernavaca 62250, Morelos, Mexico

[2] Univ Nacl Autonoma Mexico, Inst Fisiol Celular, Mexico City, DF, Mexico

来源：

FEBS LETTERS | 2003年 / 539卷 / 1-3期

关键词：

Cu; Zn-superoxide dismutase; protein kinase A pathway; protein oxidation;

D O I：

10.1016/S0014-5793(03)00199-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Here we analyzed the role of the antioxidant response in Saccharomyces cerevisiae adaptation to hyperosmotic stress. We show that Cu,Zn-superoxide dismutase (SOD1) plays a fundamental role in this adaptation process since under hyperosmosis SOD1 mutants lead to high protein oxidation levels and show a sensitive phenotype, which is reversed by the addition of N-acetylcysteine to the medium. Pretreatment with MnCl2, a superoxide scavenger, improves the survival of the sod1 strain upon hyperosmosis. Additionally, we show that upon hyperosmotic shock there is a small and transient increase in SOD1 transcript levels, regulated by the protein kinase A-cAMP and SKN7 pathways. (C) 2003 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.

引用

页码：68 / 72

页数：5

共 25 条

[1] USE OF FLUORESCEIN HYDRAZIDE AND FLUORESCEIN THIOSEMICARBAZIDE REAGENTS FOR THE FLUOROMETRIC-DETERMINATION OF PROTEIN CARBONYL GROUPS AND FOR THE DETECTION OF OXIDIZED PROTEIN ON POLYACRYLAMIDE GELS [J].