Quantitation of cholesterol incorporation into extruded lipid bilayers

被引：21

作者：

Ibarguren, Maitane ^{[1
,2
]}

Alonso, Alicia ^{[1
,2
]}

Tenchov, Boris G. ^{[3
]}

Goni, Felix M. ^{[1
,2
]}

机构：

[1] Univ Basque Country, Unidad Biofis, EHU, Ctr Mixto CSIS, E-48080 Bilbao, Spain

[2] Univ Basque Country, Dept Bioquim, E-48080 Bilbao, Spain

[3] Northwestern Univ, Dept Biochem Mol Biol & Cell Biol, Evanston, IL 60208 USA

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES | 2010年 / 1798卷 / 09期

关键词：

Cholesterol; Lipid bilayers; Inverted hexagonal phases; Phospholipid; Sphingomyelin; Extrusion vesicles; NUCLEAR-MAGNETIC-RESONANCE; DIFFERENTIAL SCANNING CALORIMETRY; MAXIMUM SOLUBILITY; MEMBRANES; MIXTURES; PHOSPHATIDYLCHOLINE; PHOSPHATIDYLETHANOLAMINE; SPHINGOMYELIN; DOMAIN; PHOSPHOLIPIDS;

D O I：

10.1016/j.bbamem.2010.06.004

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

Cholesterol incorporation into lipid bilayers, in the form of multilamellar vesicles or extruded large unilamellar vesicles, has been quantitated. To this aim, the cholesterol contents of bilayers prepared from phospholipid:cholesterol mixtures 33-75 mol% cholesterol have been measured and compared with the original mixture before lipid hydration. There is a great diversity of cases, but under most conditions the actual cholesterol proportion present in the extruded bilayers is much lower than predicted. A quantitative analysis of the vesicles is thus required before any experimental study is undertaken. (C) 2010 Elsevier B.V. All rights reserved.

引用

页码：1735 / 1738

页数：4

共 31 条

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[2]

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[3]

Molecular organization of cholesterol, in polyunsaturated membranes: Microdomain formation [J].