Cytogenetic analysis using simultaneous and sequential fluorescence in situ hybridization

被引：8

作者：

Escudero, T ^{[1
]}

Fuster, C ^{[1
]}

Coll, MD ^{[1
]}

Egozcue, J ^{[1
]}

机构：

[1] Univ Autonoma Barcelona, Fac Ciencies, Dept Biol Celular & Fisiol, Unitat Biol Cellular, Bellaterra 08193, Spain

来源：

CANCER GENETICS AND CYTOGENETICS | 1998年 / 100卷 / 02期

关键词：

D O I：

10.1016/S0165-4608(97)00029-0

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

A sequential fluorescence in situ hybridization (FISH) technique is described. This method allows the detection of up to eighteen chromosome pairs in consecutive hybridizations (8 steps) on the same metaphase using centromeric, whole chromosome painting, and single copy DNA probes with different fluorochromes, The technique may be used with diagnostic purposes in cases with poor cytogenetic material. (C) Elsevier Science Inc., 1998.

引用

页码：111 / 113

页数：3

共 5 条

[1] REPROBING OF DNA-DNA INSITU HYBRIDIZATION PREPARATIONS
HESLOPHARRISON, JS
HARRISON, GE
LEITCH, IJ
[J]. TRENDS IN GENETICS, 1992, 8 (11) : 372 - 373
[2] One FISH, two FISH, red FISH, blue FISH
LeBeau, MM
[J]. NATURE GENETICS, 1996, 12 (04) : 341 - 344
[3] Multicolor spectral karyotyping of human chromosomes
Schrock, E
duManoir, S
Veldman, T
Schoell, B
Wienberg, J
FergusonSmith, MA
Ning, Y
Ledbetter, DH
BarAm, I
Soenksen, D
Garini, Y
Ried, T
[J]. SCIENCE, 1996, 273 (5274) : 494 - 497
[4] Karyotyping human chromosomes by combinatorial multi-fluor FISH
Speicher, MR
Ballard, SG
Ward, DC
[J]. NATURE GENETICS, 1996, 12 (04) : 368 - 375
[5] Rehybridization on metaphases studied previously by FISH - An approach to analyze chromosome aberrations
Wang, MR
Perissel, B
Malet, P
[J]. CANCER GENETICS AND CYTOGENETICS, 1995, 85 (01) : 58 - 60

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