Production of immunoreactive thyroglobulin C-terminal fragments during thyroid hormone synthesis

被引：20

作者：

Duthoit, C ^{[1
]}

Estienne, V ^{[1
]}

Delom, F ^{[1
]}

Durand-Gorde, JM ^{[1
]}

Mallet, B ^{[1
]}

Carayon, P ^{[1
]}

Ruf, J ^{[1
]}

机构：

[1] Univ Aix Marseille 2, Fac Med Timone, French Inst Hlth & Med Res, U38, Marseille, France

来源：

ENDOCRINOLOGY | 2000年 / 141卷 / 07期

关键词：

D O I：

10.1210/en.141.7.2518

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Here, we studied the fragmentation of the prothyroid hormone, ehyroglobulin (Tg), which occurs during thyroid hormone synthesis, a process which involves iodide, thyroperoxidase, and the H2O2-generating system, consisting of glucose and glucose oxidase. Various peptides were found to be immunoreactive to autoantibodies to Tg from patients and monoclonal antibodies directed against the immunodominant region of Tg. The smallest peptide (40 kDa) bore thyroid hormones and was identified at the C-terminal end of the Tg molecule, which shows homologies with acetylcholinesterase. Similar peptides were obtained by performing metal-mediated oxidation of Tg via a Fenton reaction. It was concluded that the oxidative stress induced during hormone synthesis generates free radicals, which, in turn, cleave Tg into immunoreactive peptides.

引用

页码：2518 / 2525

页数：8

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