Molecular analysis of Mycobacterium tuberculosis DNA from a family of 18th century Hungarians

被引:55
作者
Fletcher, HA
Donoghue, HD
Taylor, GM
van der Zanden, AGM
Spigelman, M
机构
[1] UCL, Dept Med Microbiol, London W1T 4JF, England
[2] Univ London Imperial Coll Sci Technol & Med, Ctr Microbiol & Infect, London SW7 2AZ, England
[3] Gelre Hosp, NL-7300 DS Apeldoorn, Netherlands
来源
MICROBIOLOGY-SGM | 2003年 / 149卷
关键词
D O I
10.1099/mic.0.25961-0
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The naturally mummified remains of a mother and two daughters found in an 18th century Hungarian crypt were analysed, using multiple molecular genetic techniques to examine the epidemiology and evolution of tuberculosis. DNA was amplified from a number of targets on the Mycobacterium tuberculosis genome, including DNA from IS6110, gyrA, katG codon 463, oxyR, dnaA-dnaN, mtp40, plcD and the direct repeat (DR) region. The strains present in the mummified remains were identified as M. tuberculosis and not Mycobacterium bovis, from katG and gyrA genotyping, PCR from the oxyR and mtp40 loci, and spoligotyping. Spoligotyping divided the samples into two strain types, and screening for a deletion in the MT1801-plcD region initially divided the strains into three types. Further investigation showed, however, that an apparent deletion was due to poor DNA preservation. By comparing the effect of PCR target size on the yield of amplicon, a clear difference was shown between 18th century and modern M. tuberculosis DNA. A two-centre system was used to confirm the findings of this study, which clearly demonstrate the value of using molecular genetic techniques to study historical cases of tuberculosis and the care required in drawing conclusions. The genotyping and spoligotyping results are consistent with the most recent theory of the evolution and spread of the modern tuberculosis epidemic.
引用
收藏
页码:143 / 151
页数:9
相关论文
共 50 条
[1]   Mycobacterium tuberculosis complex DNA in ancient human bones [J].
Baron, H ;
Hummel, S ;
Herrmann, B .
JOURNAL OF ARCHAEOLOGICAL SCIENCE, 1996, 23 (05) :667-671
[2]   RAPID AND SIMPLE METHOD FOR PURIFICATION OF NUCLEIC-ACIDS [J].
BOOM, R ;
SOL, CJA ;
SALIMANS, MMM ;
JANSEN, CL ;
WERTHEIMVANDILLEN, PME ;
VANDERNOORDAA, J .
JOURNAL OF CLINICAL MICROBIOLOGY, 1990, 28 (03) :495-503
[3]   DNA from Mycobacterium-tuberculosis complex identified in North American, pre-Columbian human skeletal remains (Archaeology and human biology) [J].
Braun, M ;
Cook, DC ;
Pfeiffer, S .
JOURNAL OF ARCHAEOLOGICAL SCIENCE, 1998, 25 (03) :271-277
[4]   Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence [J].
Cole, ST ;
Brosch, R ;
Parkhill, J ;
Garnier, T ;
Churcher, C ;
Harris, D ;
Gordon, SV ;
Eiglmeier, K ;
Gas, S ;
Barry, CE ;
Tekaia, F ;
Badcock, K ;
Basham, D ;
Brown, D ;
Chillingworth, T ;
Connor, R ;
Davies, R ;
Devlin, K ;
Feltwell, T ;
Gentles, S ;
Hamlin, N ;
Holroyd, S ;
Hornby, T ;
Jagels, K ;
Krogh, A ;
McLean, J ;
Moule, S ;
Murphy, L ;
Oliver, K ;
Osborne, J ;
Quail, MA ;
Rajandream, MA ;
Rogers, J ;
Rutter, S ;
Seeger, K ;
Skelton, J ;
Squares, R ;
Squares, S ;
Sulston, JE ;
Taylor, K ;
Whitehead, S ;
Barrell, BG .
NATURE, 1998, 393 (6685) :537-+
[5]   Identification of Mycobacterium DNA in an Egyptian Pott's disease of 5,400 years old [J].
Crubézy, E ;
Ludes, B ;
Poveda, JD ;
Clayton, J ;
Crouau-Roy, B ;
Montagnon, D .
COMPTES RENDUS DE L ACADEMIE DES SCIENCES SERIE III-SCIENCES DE LA VIE-LIFE SCIENCES, 1998, 321 (11) :941-951
[6]  
Dale JW, 2001, INT J TUBERC LUNG D, V5, P216
[7]   MYCOBACTERIUM-BOVIS INFECTIONS IN SAN-DIEGO - A CLINICOEPIDEMIOLOGIC STUDY OF 73 PATIENTS AND A HISTORICAL REVIEW OF A FORGOTTEN PATHOGEN [J].
DANKNER, WM ;
WAECKER, NJ ;
ESSEY, MA ;
MOSER, K ;
THOMPSON, M ;
DAVIS, CE .
MEDICINE, 1993, 72 (01) :11-37
[8]   Multiprimer PCR system for differential identification of mycobacteria in clinical samples [J].
DelPortillo, P ;
Thomas, MC ;
Martinez, E ;
Maranon, C ;
Valladares, B ;
Patarroyo, ME ;
Lopez, MC .
JOURNAL OF CLINICAL MICROBIOLOGY, 1996, 34 (02) :324-328
[9]   AMPLIFICATION OF A SPECIES-SPECIFIC DNA FRAGMENT OF MYCOBACTERIUM-TUBERCULOSIS AND ITS POSSIBLE USE IN DIAGNOSIS [J].
DELPORTILLO, P ;
MURILLO, LA ;
PATARROYO, ME .
JOURNAL OF CLINICAL MICROBIOLOGY, 1991, 29 (10) :2163-2168
[10]  
Donoghue HD, 1998, LETT APPL MICROBIOL, V27, P265