Rat fetuin:: distribution of protein and mRNA in embryonic and neonatal rat tissues

被引:44
作者
Terkelsen, OBF
Jahnen-Dechent, W
Nielsen, H
Moos, T
Fink, E
Nawratil, P
Müller-Esterl, W
Mollgård, K
机构
[1] Univ Mainz, Inst Physiol Chem & Pathobiochem, D-55099 Mainz, Germany
[2] Univ Copenhagen, Panum Inst, Inst Med Anat, DK-2200 Copenhagen N, Denmark
[3] Univ Copenhagen, Panum Inst, Inst Med Biochem & Genet, DK-2200 Copenhagen N, Denmark
[4] Univ Munich, Dept Clin Chem & Clin Biochem, D-8000 Munich, Germany
来源
ANATOMY AND EMBRYOLOGY | 1998年 / 197卷 / 02期
关键词
blood proteins; alpha-globulins; fetal development; cell differentiation; embryo;
D O I
10.1007/s004290050124
中图分类号
R602 [外科病理学、解剖学]; R32 [人体形态学];
学科分类号
100101 ;
摘要
Fetuin is a serum protein widely distributed in the animal kingdom and found in all mammalian species so far investigated. It is mainly a fetal protein, in the sense that the highest concentrations are found in serum and body fluids of embryos and fetuses. In order to elucidate possible biological functions of fetuin, we have studied its synthesis and distribution during the prenatal development of the rat with immunohistochemistry and in situ hybridization. We have isolated fetuin from rat serum and produced an antibody against this protein. In situ hybridization was performed using a 375-nucleotides-long digoxigenin-labeled riboprobe. Fetuin was unevenly distributed in all organ systems during development, with the most pronounced expression at E 10Fetuin is a serum protein widely distributed in the animal kingdom and found in all mammalian species so far investigated. It is mainly a fetal protein, in the sense that the highest concentrations are found in serum and body fluids of embryos and fetuses. In order to elucidate possible biological functions of fetuin, we have studied its synthesis and distribution during the prenatal development of the rat with immunohistochemistry and in situ hybridization. We have isolated fetuin from rat serum and produced an antibody against this protein. In situ hybridization was performed using a 375-nucleotides-long digoxigenin-labeled riboprobe. Fetuin was unevenly distributed in all organ systems during development, with the most pronounced expression at E16-E18. Fetuin expression was present in germinal cell populations, e.g., in the basal layer in the skin, in the germinal cell populations in the brain anlage and the gonads, and it was heavily expressed in the fetal hemopoietic liver. Furthermore, fetuin was expressed in the gastrointestinal epithelium prior to the development of glands and crypts. Fetuin was widely distributed in mesenchymal derived tissues, e.g., bone and muscle. In the developing kidney fetuin was heavily expressed is both mesenchymal condensations and glomerular anlages. Thus, fetuin was located in cells or structures undergoing differentiation and transformation. As fetuin has been shown previously to interfere with hormone signaling of transforming growth factor-beta, insulin and hepatocyte-growth factor, fetuin might be involved in cell differentiation and tissue transformation during the initial histogenesis, i.e., the time period in which cellular phenotypic characteristics are established.
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页码:125 / 133
页数:9
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