cDNA-AFLP display for the isolation of Peronospora parasitica genes expressed during infection in Arabidopsis thaliana

被引：49

作者：

van der Biezen, EA ^{[1
]}

Juwana, H ^{[1
]}

Parker, JE ^{[1
]}

Jones, JDG ^{[1
]}

机构：

[1] John Innes Ctr Plant Sci Res, Sainsbury Lab, Norwich NR4 7UH, Norfolk, England

来源：

MOLECULAR PLANT-MICROBE INTERACTIONS | 2000年 / 13卷 / 08期

关键词：

differential display;

D O I：

10.1094/MPMI.2000.13.8.895

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

To identify genes from the obligatory biotrophic oomycete Peronospora parasitica that are expressed during infection in Arabidopsis thaliana we employed cDNA-amplified fragment length polymorphism (AFLP) display. cDNA-AFLP fragments from infected and non-infected leaves were separated in parallel by gel electrophoresis and displayed by autoradiography, Most differential gene fragments were derived from P. parasitica.

引用

页码：895 / 898

页数：4

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