Sequencing of variable regions of the 16S rRNA gene for identification of lactic acid bacteria isolated from the intestinal microbiota of healthy salmonids

被引：84

作者：

Luis Balcazar, Jose ^{[1
]}

de Blas, Ignacio ^{[1
]}

Ruiz-Zarzuela, Imanol ^{[1
]}

Vendrell, Daniel ^{[1
]}

Girones, Olivia ^{[1
]}

Luis Muzquiz, Jose ^{[1
]}

机构：

[1] Univ Zaragoza, Fac Vet Sci, Lab Fish Pathol, E-50013 Zaragoza, Spain

来源：

COMPARATIVE IMMUNOLOGY MICROBIOLOGY AND INFECTIOUS DISEASES | 2007年 / 30卷 / 02期

关键词：

isolation; salmonids; lactic acid bacteria; identification; DNA sequencing; 16S rRNA gene; variable regions; LACTOBACILLI; FISH;

D O I：

10.1016/j.cimid.2006.12.001

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The aim of this study was to identify lactic acid bacteria (LAB) using polymerase chain reaction (PCR) amplification of variable regions of the 16S rRNA gene. Thirteen LAB strains were isolated from the intestinal microbiota of healthy salmonids. A similar to 500-bp region of the highly conserved 16S rRNA gene was PCR-amplified and following this, a portion of the amplicon (272-bp) including the V1 and V2 variable regions was sequenced. The sequence containing both the V1 and V2 region provided strong evidence for the identification of LAB. The LAB strains were identified as Camobacteriton maltaroinaticum, Lactobacillus curvatus, Lactobacillus sakei, Lactobacillits plantarum, Lactococcus lactis subsp. cremoris, Lactococcus lactis subsp. lactis, and Leuconostoc mesenteroides. The method described was found to be a very simple, rapid, specific, and low-cost tool for the identification of unknown strains of LAB. (c) 2007 Elsevier Ltd. All rights reserved.

引用

页码：111 / 118

页数：8

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